文献类型：期刊文献

英文题名：Effects of 3,4-Dichloroaniline on Testicle Enzymes as Biological Markers in Rats

作者：Zhang, Bo[1];Lin, Sen[1]

第一作者：张斌;张波

通讯作者：Zhang, B[1]

机构：[1]Beijing Union Univ, Coll Appl Sci & Humanities, Dept Biol, Beijing 100083, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Appl Sci & Humanities, Dept Biol, Beijing 100083, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2009

卷号：22

期号：1

起止页码：40-43

外文期刊名：BIOMEDICAL AND ENVIRONMENTAL SCIENCES

收录：;Scopus(收录号：2-s2.0-65349154782);WOS:【SCI-EXPANDED(收录号:WOS:000263776100007)】；

基金：This work was supported by the Natural Science Foundation of Beijing (No. 7063071).

语种：英文

外文关键词：3, 4-dichloraniline; Rat; Marker testicular enzymes

摘要：Objective To investigate the effects of 3,4-dichloroaniline (3,4-DCA) on activities of testicle enzymes as biological markers in rats. Methods Fifty male rats were randomly divided into 5 groups (n=10). One group was left untreated and used as a solvent control (administered orally by corn oil), while the other 4 groups were treated with 3, 4-DCA. Corn oil was used as a solvent, and 3,4-DCA was diluted into tested concentrations (39, 81, 170, and 357 mg/kg). All the groups orally administered 3,4-DCA or corn oil, once a day for 4 weeks. The testicle tissue was homogenized in a 0.1 mol/L potassium phosphate buffer (0.1 mol/L, pH 7.2). The crude homogenate was centrifuged at 6000 rpm for 5 min at 4 degrees C. The supernatant obtained was used as an enzyme extract for determination of the enzyme activities. Results Compared with the control, the activities of ALP, ACP, and SDH were increased significantly at a lower level of 3,4-DCA, and decreased at a higher level of 3, 4-DCA, whreas the activities of LDH, LDH-X, and G6PDH were inhibited significantly with the increased 3A-DCA concentration. Organ coefficient "organ weight/total body weight x 100" of testis, liver. and spleen increased significantly with the increased 3A-DCA concentration. These results suggest that 3A-DCA toxicity to the male reproductive system was associated with the activities of testicular enzymes which are the sensitive biochemical endpoints reflecting 3,4-DCA toxicity to the male reproductive system. Conclusion 3,4-DCA has toxicity to the reproductive system in male rats.