文献类型：期刊文献

中文题名：双蛋白对大鼠血脂的影响研究

英文题名：Study on the effect of double protein on blood lipid in rats

作者：闫文杰[1,2];松伟[1];韩迪[2];李琥[2];冯朵[2];王靖[2]

第一作者：闫文杰

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]农业农村部食物与营养发展研究所,北京100081

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：13

期号：24

起止页码：7934-7940

中文期刊名：食品安全质量检测学报

外文期刊名：Journal of Food Safety and Quality

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：中国农业科学院农业科技创新工程项目(CAAS-ASTIP-2022-IFND)。

语种：中文

中文关键词：双蛋白;血脂异常;作用机制

外文关键词：double protein;dyslipidemia;mechanism of action

摘要：目的探讨双蛋白对高脂模型大鼠血脂影响及作用机制。方法饲喂高脂饲料制备高脂模型,造模后根据血液生化指标分为空白组、高脂模型组、双蛋白低剂量组(0.83 g/kg)、双蛋白中剂量组(1.67 g/kg)、双蛋白高剂量组(5.00 g/kg)5组。连续灌胃6周后处死,检测胆固醇(total cholesterol,TC)、甘油三酯(total glyceride,TG)、低密度脂蛋白胆固醇(lowdensitylipoprotein,LDL-C)、高密度脂蛋白胆固醇(highdensitylipteincholesterol,HDL-C)。酶联免疫吸附测定乙酰辅酶A羧化酶(acetyl-CoA carboxylase,ACC)、胰高血糖素(glucagon,GC)、胰岛素(insulin,INS)、3-羟基-3-甲酰基-CoA还原酶(3-hydroxy-3-methyl glutaryl coenzyme A reductase,HMG-CoA)含量。结果与空白组比较,模型组除HMG-CoA外其他指标均有显著差异(P<0.05);与模型组相比,高剂量组TG、LDL-C、TC含量显著下降(P<0.01、P<0.05),GC、HDL-C、INS含量显著提升(P<0.05、P<0.01),各组HMG-CoA水平无显著差异。结论双蛋白可降低模型大鼠血脂水平,具有降低血脂的功能。其作用机制可能与调节GC、ACC等水平有关。
Objective To explore the effect and mechanism of double protein on blood lipid in hyperlipidemic model rats.Methods The high-fat model was made by feeding high-fat diet.After the model was made,it was divided into 5 groups according to blood biochemical indexes:Blank group,high-fat model group,double protein low-dose group(0.83 g/kg),double protein medium dose group(1.67 g/kg)and double protein high-dose group(5.00 g/kg).After continuous gavage for 6 weeks,the rats were killed and the total cholesterol(TC),total glyceride(TG),low density lipoprotein(LDL-C)and high density liptein cholesterol(HDL-C)were measured.The content of acetyl-CoA carboxylase(ACC),glucagon(GC),insulin(INS)and 3-hydroxy-3-methyl glutaryl coenzyme A reductase(HMG-CoA)was determined by enzyme linked immunosorbent assay.Results Compared with the blank group,the model group had significant differences in other indicators except HMG-CoA(P<0.05).Compared with the model group,the content of TG,LDL-C and TC in the high-dose group decreased significantly(P<0.01,P<0.05),and the content of GC,HDL-C and INS increased significantly(P<0.05,P<0.01).There was no significant difference in HMG-CoA levels among the groups.Conclusion The double protein can reduce the blood lipid level of model rats and has the function of reducing blood lipid.The mechanism of action may be related to the regulation of GC and ACC levels.