文献类型：期刊文献

英文题名：Cholesterol-lowing effect of taurine in HepG2 cell

作者：Guo, Junxia[1,2];Gao, Ya[2];Cao, Xuelian[2];Zhang, Jing[1,2];Chen, Wen[1,2]

第一作者：郭俊霞

通讯作者：Chen, W[1];Chen, W[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Fatou West 18#, Beijing 100023, Peoples R China;[2]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China

第一机构：北京联合大学生物化学工程学院食品科学系

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Dept Food Sci, Fatou West 18#, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;[11417146]北京联合大学生物化学工程学院食品科学系;

年份：2017

卷号：16

期号：1

外文期刊名：LIPIDS IN HEALTH AND DISEASE

收录：;Scopus(收录号：2-s2.0-85015407567);WOS:【SCI-EXPANDED(收录号:WOS:000396787800002)】；

基金：This work was supported by grants from the National Natural Science Foundation for Young Scholars of China (31401501) and Natural Science Foundation of Beijing Municipality (5142004). The content is solely the responsibility of the authors.

语种：英文

外文关键词：Taurine; Cholesterol; Bile acids; HepG2 cell

摘要：Background: A number of studies indicate that taurine promotes cholesterol conversion to bile acids by upregulating CYP7A1 gene expression. Few in vitro studies are concerned the concentration change of cholesterol and its product of bile acids, and the molecular mechanism of CYP7A1 induction by taurine. Methods: The levels of intracellular total cholesterol (TC), free cholesterol (FC), cholesterol ester (EC), total bile acids (TBA) and medium TBA were determined after HepG2 cells were cultured for 24/48 h in DMEM supplemented with taurine at the final concentrations of 1/10/20 mM respectively. The protein expressions of CYP7A1, MEK1/2, c-Jun, p-c-Jun and HNF-4 alpha were detected. Results: Taurine significantly reduced cellular TC and FC in dose -and time-dependent ways, and obviously increased intracellular/medium TBA and CYP7A1 expressions. There was no change in c-Jun expression, but the protein expressions of MEK1/2 and p-c-Jun were increased at 24 h and inhibited at 48 h by 20 mM taurine while HNF4 alpha was induced after both of the 24 h and 48 h treatment. Conclusion: Taurine could enhance CYP7A1 expression by inducing HNF4a and inhibiting MEK1/2 and p-c-Jun expressions to promote intracellular cholesterol metabolism.