文献类型：期刊文献

英文题名：Identification of Small Biomolecular Compounds by MALDI-TOF-Mass Spectrometry

作者：Huo, Qing[1];Liu, Jianan[2];Xiong, Shaoxiang[2];Shi, Rongjian[3]

第一作者：霍清

通讯作者：Liu, JA[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China;[3]Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China.

年份：2013

卷号：25

期号：18

起止页码：10137-10143

外文期刊名：ASIAN JOURNAL OF CHEMISTRY

收录：;Scopus(收录号：2-s2.0-84901854982);WOS:【SCI-EXPANDED(收录号:WOS:000332937600023)】；

基金：This work was supported by Institute of Chemistry, Chinese Academy of Sciences.

语种：英文

外文关键词：Matrix-assisted laser ionization; Sensitivity; Matrix; Molecular weight

摘要：Matrix assisted laser desorption ionization time of flight mass spectrometry MALDI-TOF-MS was generally used to analyze macromolecular compounds. In this paper we study how to use the MALDI to determine the molecular weight of small molecule compounds, such as sugars, amino acids, clenbuterol, diphenylmethyl amines, plant hormones, etc. We also studied how to exploring the sensitivity of matrix and how to application the sensitivity. We find a macromolecular 1,2,3,4-tetrakis(3',4'-hydroxyphenyl)-thiophene as matrix, it not only absorbs the laser energy but also makes sample desorption ionization. At the same time it does not produce interfering ions to low molecular weight. In addition, perfluoro C-60 was added in matrix sensitivity can be improved.