文献类型：期刊文献

中文题名：不同物质组成餐厨垃圾的多酶协同酶解研究

英文题名：SYNERGISTIC ENZYMATIC HYDROLYSIS OF KITCHEN WASTE WITH DIFFERENT COMPONENTS

作者：邱卫华[1];修涵[1,2];赵芸卿[1,2];姜慧[2]

第一作者：邱卫华

机构：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190;[2]北京联合大学旅游学院餐饮管理系,北京100101

第一机构：中国科学院过程工程研究所生化工程国家重点实验室,北京100190

年份：2023

卷号：41

期号：S01

起止页码：383-387

中文期刊名：环境工程

外文期刊名：Environmental Engineering

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：北京市自然科学基金(J210013);北京高等学校高水平人才交叉培养“实培计划”大学生毕业设计(科研类)项目

语种：中文

中文关键词：餐厨垃圾;协同酶解;糖化效率;成本分析;定向生物转化

外文关键词：kitchen waste;enzyme-cocktail;synergistically enzymatic hydrolysis;saccharification efficiency;oriented bioconversion technical

摘要：餐厨垃圾的酶解效率受其物质组成复杂性与差异性的制约。研究了果胶酶(PEC)、纤维素酶(CEL)和糖化酶(GLU)组成的多酶复合体系对高淀粉餐厨垃圾(HSKW)和高油高盐餐厨垃圾(HOHSKW)酶解糖化的差异。对于HSKW,以10 U/g CEL和24 U/g PEC预水解后协同390 U/g GLU水解时,还原糖得率(Y_(RS))达到最高值(739.74±20.16)mg/g TS,是910 U/g GLU单独水解的1.03倍。对于HOHSKW,以20 U/g CEL和24 U/g PEC预水解后协同520 U/g GLU水解时,Y_(RS)达到最高值(582.48±20.43)mg/g TS,是1300 U/g GLU单独水解的1.90倍。成本估算表明,HSKW和HOHSKW经多酶混合物协同水解所生产还原糖的酶成本较单独GLU水解可分别降低27.4%和60.7%。由此可见,多酶协同水解显著降低了餐厨垃圾生产可发酵糖的酶成本,从而提高了后续餐厨垃圾绿色定向生物转化的经济技术可行性。
As a solid-phase heterogeneous material,enzymatic hydrolysis is a key technology for the bioconversion of kitchen waste(KW),but restricted by the complexity and difference of its components.The saccharification of high starch content kitchen waste(HSKW)and high oil and high salt content kitchen waste(HOHSKW)catalyzed by the enzyme cocktail composing of pectinase(PEC),cellulase(CEL)and glucoamylase(GLU)were investigated in this paper.For HSKW,when pre-hydrolyzed with 10 U/g CEL and 24 U/g PEC and then synergistically hydrolyzed with 390 U/g GLU,the highest reducing sugar yield(Y_(RS))of 739.74±20.16 mg/g TS was obtained,which was 1.03 times of that hydrolyzed with 910 U/g GLU alone.As for HOHSKW,the highest Y_(RS)of 582.48±20.43 mg/g TS was obtained when pre-hydrolyzed with 20 U/g CEL and 24 U/g PEC followed by synergistic hydrolysis with 520 U/g GLU,which was 1.90 times of that hydrolyzed with 1300 U/g GLU.The enzyme cost estimation indicated that compared to hydrolyzing with GLU alone the enzyme cost of producing reducing sugar from HSKW and HOHSKW by hydrolyzing with the enzyme cocktail of PEC,CEL and GLU were decreased by 27.4%and 60.7%,respectively.It can be concluded that the synergistic hydrolysis of CEL,PEC and GLU significantly reduces the enzyme cost of producing fermentable sugars from kitchen waste,thereby improving the economic and technical feasibility of the subsequent green and oriented bioconversion of kitchen waste.