文献类型：期刊文献

英文题名：Response Mechanism of Lactobacillus plantarum under Simulated Digestion Based on Proteomics and Metabolomics Analysis

作者：Zhao, L.[1];Cheng, Z.[1];Ling, Y.[1];Wu, Y.[1];Zhang, M.[2,3];Yang, H.[1];Li, Z.[1]

第一作者：赵龙

通讯作者：Li, Z[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Univ Manitoba, Dept Phys & Astron, Winnipeg, MB R3T 2N2, Canada;[3]Sino Canada Hlth Engn Res Inst, Hefei 230088, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2025

外文期刊名：APPLIED BIOCHEMISTRY AND MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-105025962353);WOS:【SCI-EXPANDED(收录号:WOS:001647490100001)】；

基金：This research has been carried out by Natural Science Foundation of Beijing Municipality, grant number 6173033; Academic Research Projects of Beijing Union University, grant number ZK20202307 and Sino Canada Health Engineering Research Institute Cooperation Projects, grant number 50304JA1901.

语种：英文

外文关键词：Lactobacillus plantarum; metabolomics; proteomics; simulation digestion; response mechanism

摘要：Proteomics and a metabolomics strategy were used to investigate the probiotic properties of Lactobacillus plantarum BW2013 under simulated in vitro digestion. Thirty seven significant differential proteins including 6-phosphogluconate dehydrogenase, pyruvate kinase, F0F1 ATP synthase, ABC-type transporters, ribosomal and universal stress proteins and 40 significant differential metabolites, such as indole-3-lactic acid, Pro, trans-cinnamic acid, betaine, citric acid, D-fructose, and gamma-aminobutyric acid were identified and characterized. The association analysis indicated that most upregulated proteins and metabolites are involved primarily in stress response, regulation of intestinal flora and accumulation of beneficial substances relating to carbohydrate and energy metabolic pathways, amino acid metabolism pathways, nucleotide metabolism pathways, overall/universal stress response. The results provide new insights into the response mechanism of L. plantarum BW2013 under the simulated digestion conditions, facilitating the exploitation of its potential application in probiotic products.