文献类型：期刊文献

英文题名：Enhancement of lutein biosynthesis in chlorella vulgaris-1068 under low temperature and luminosity stress conditions

作者：Wang, Wanqing[1,2]; Chen, Mingyang[1]; Liu, Jie[1]; Hua, Wei[1,2]; Wu, Shuang[1,2]; Diao, Mengjie[1]; Cheng, Yanling[1,2]

第一作者：Wang, Wanqing;王晚晴

通讯作者：Chen, Mingyang

机构：[1] Biochemical Engineering College, Beijing Union University, Beijing, 100023, China; [2] Beijing Key Laboratory of Biomass Waste Resource Utilization, Beijing, 100023, China

第一机构：北京联合大学生物化学工程学院

年份：2017

卷号：26

期号：3

起止页码：151-156

外文期刊名：International Agricultural Engineering Journal

收录：EI(收录号：20174904494982);Scopus(收录号：2-s2.0-85036480242)

基金：This work was supported by grants from the science and technology foundation of Beijing municipal education committee and the opening foundation of Beijing key laboratory of biomass waste resource utilization to WW. We gracefully acknowledge financial support of Scientific Research Project from Facing Characteristic Discipline of Beijing Union University KYDE40201704. WW and YC conceived the research; WW and YC designed the experiments; WW, MC, JL and MD performed the research; WW, WH and YC analyzed data; and WW wrote the paper.

语种：英文

外文关键词：Algae - Biochemistry - Biosynthesis - Gene expression - Growth rate - Light - Luminance - Microorganisms - Photons - Pigments

摘要：Lutein, a high-value primary carotenoid with a broad range of applications in food, feed, nutraceutical, and pharmaceutical industries, has been widely studied in recent years. The green microalgae Chlorella vulgaris-1068(C.v-1068) screened by our lab showed both a high content in this carotenoid and a high growth rate and has a good profile for lutein production. This work studies the variety of environmental stimulants which enhance cellular accumulation of lutein from C.v-1068. The lutein accumulation and its biosynthesis pathway genes expression were analyzed under low temperature and high luminosity. We find that both cold and high light stresses can induce lutein biosynthesis in C.v-1068. In addition, during dark to light transition also leads to higher lutein productivity and the maximal lutein content (2.86 mg/g DW) was attained after six days dark-cultivated then exposed to 1000 μ photons m-2 s-1 for two days treatment. Consistently, the transcript levels of lutein biosynthesis genes PSY and ZDS were largely enhanced by cold, high light and dark to light transition treatments. And except PSY and ZDS, 1000 μ photons m-2 s-1 luminosity stress also highly increased expression of PDS as compared with low irradiance condition.