文献类型：期刊文献

中文题名：基于网络药理学和体外试验探讨巴戟天寡糖抗抑郁作用机制

英文题名：Antidepressant Mechanism of Morinda officinalis Oligosaccharides Based on Network Pharmacology and in Vitro Experiment

作者：王春羽[1];戴雪伶[1];刘彦霞[1]

第一作者：王春羽

机构：[1]北京联合大学生物化学工程学院,北京100023

第一机构：北京联合大学生物化学工程学院

年份：2026

卷号：26

期号：4

起止页码：60-70

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute of Food Science and Technology

收录：;EI(收录号：20262420926288);北大核心:【北大核心2023】；

语种：中文

中文关键词：巴戟天寡糖;抗抑郁;体外试验;炎症反应;药食同源

外文关键词：Morinda officinalis oligosaccharides;antidepressant;in vitro experiment;inflammatory reaction;homology of medicine and food

摘要：目的:采用网络药理学方法探讨巴戟天寡糖(MOO)的抗抑郁潜在作用机制,并通过体外试验验证。方法:通过TCMSP等数据库结合文献检索筛选出MOO的主要活性成分;基于GeneCards、Swiss Target数据库获取活性成分及抑郁症相关的靶点,再将其取交集得到交集靶点;将交集靶点导入STRING数据库构建蛋白质-蛋白质相互作用(PPI)网络,对MOO抗抑郁潜在作用靶点及相关通路进行预测。通过体外培养小鼠BV2小胶质细胞,探究MOO对BV2细胞PI3K/Akt通路的影响。结果:得到MOO活性成分共3个,包括奈斯糖、1F-果呋喃糖基耐斯糖和蔗果七糖,对应作用靶点35个;抑郁症靶点8154个,与活性成分靶点取交集后得到共有靶点32个;KEGG通路富集分析显示基因集显著富集于PI3K/Akt信号通路、癌症机制研究等。体外试验表明,脂多糖(LPS)处理可使BV2细胞炎症水平升高,PI3K和Akt基因在转录水平下调且P-PI3K/PI3K、P-Akt/Akt的相对表达水平明显下调,而MOO预保护能够降低LPS对BV2细胞的促炎作用,并上调PI3K和Akt基因及P-PI3K/PI3K、P-Akt/Akt的相对表达水平,暗示MOO的抗抑郁机制与PI3K/Akt通路相关,与网络药理学结果相符。结论:MOO可以通过多组分、多靶点、多途径协同发挥抗抑郁作用,其机制可能与炎症反应及PI3K/Akt信号转导等过程密切相关,这为药食同源保健食品的开发提供理论基础。
Objectives:The purpose of this study is to explore the potential antidepressant mechanism of Morinda officinalis oligosaccharides(MOO)by network pharmacology,and to verify it by in vitro experiments.Methods:The main active components of MOO were screened out through databases such as TCMSP and literature retrieval.Active components and targets related to depression were obtained based on GeneCards and Swiss Target databases,and then the intersection targets were obtained.The protein-protein interaction(PPI)network was constructed by importing the intersection targets into the STRING database,and the potential targets and related pathways of MOO antidepressant were predicted.By culturing mouse BV2 microglia in vitro,the effect of MOO on the PI3K/Akt pathway in BV2 cells were explored.Results:Three active components of MOO were obtained,including nystose,1F-Fructofuranosylnystose,and heptasaccharide,with 35 corresponding targets.There were 8154 targets for depression,and 32 common targets were obtained after intersection with active components targets;KEGG pathway enrichment analysis showed that the gene set was significantly enriched in PI3K/Akt signaling pathway,cancer mechanism research and so on.In vitro experiments showed that lipopolysaccharide(LPS)treatment could increase the inflammatory level of BV2 cells,and the PI3K and Akt genes were down-regulated at the transcription level,and the relative expression levels of P-PI3K/PI3K and P-Akt/Akt were obviously down-regulated,while MOO pre-protection could reduce the pro-inflammatory effect of LPS on BV2 cells.The relative expression levels of PI3K and Akt genes and P-PI3K/PI3K and P-Akt/Akt were up-regulated,suggesting that the antidepressant mechanism of MOO is related to PI3K/Akt pathway,which is consistent with the results of network pharmacology.Conclusions:MOO plays an antidepressant role through multi-components,multi-targets and multi-channels,and its mechanism may be closely related to inflammatory reaction and PI3K/Akt signal transduction,which provides a theoretical basis for the development of health food with homology of medicine and food.