文献类型：期刊文献

英文题名：Efficient and environmental friendly extraction and purification of lutein from wet microalgae chlorella vulgaris

作者：Xu, Yingying[1]; Wang, Wanqing[1,2]; Liu, Wenhui[1,2]; Tian, Chaoyu[1]; Hua, Wei[1,2]; Cheng, Yanling[1,2]

第一作者：Xu, Yingying

通讯作者：Cheng, Yanling

机构：[1] Biochemical Engineering College, Beijing Union University, Beijing, 100023, China; [2] Beijing Key Laboratory of Biomass Waste Resource Utilization, Beijing, 100023, China

第一机构：北京联合大学生物化学工程学院

年份：2016

卷号：25

期号：2

起止页码：182-192

外文期刊名：International Agricultural Engineering Journal

收录：EI(收录号：20163602772212);Scopus(收录号：2-s2.0-84984637761)

基金：This program was supported by Beijing Municipal Education Commission (KZ20141417038); Ministry of Science and Technology, China "863 Plan" (2014AA022002,2015AA020200); International cooperation program (2014DFA61040, 2015DFA60170).

语种：英文

外文关键词：Algae - Ethanol - Hexane - Microorganisms - Optimization - Potassium hydroxide - Purification - Surface properties - Ultrasonic applications

摘要：In this paper, a method composed of ultrasonic-assisted ethanol extraction, saponification, and hexane extraction for lutein recovery was developed. The extraction process was optimized by using single factorial experiment design and response surface methodology. Central Composite Design was applied to evaluate the effects of three independent variables. The optimum ethanol extraction conditions were as follows: extraction temperature 51.07°C, extraction time 11.95 min, and ratio of liquid to material 514.78:1 mL/g. Under optimal conditions, the experimental lutein yield was 204.70±3.68 mg/g, which corresponded well with the predicted yield. And then, 4% KOH solution was added by the ratio of 1:10 to the crude extracts for 20 min under room temperature. Finally, hexane was used to recover the lutein from the reaction mixture. These results indicate that this method has the potential to be a scaleable and industrially applicable technique for lutein production.