文献类型：期刊文献

英文题名：A Novel Cationic Ribonuclease with Antimicrobial Activity from Rana dybowskii

作者：Tao, Fengyun[1,2];Fan, Meiyin[1];Zhao, Wei[1];Lin, Qiang[1];Ma, Runyu[2]

第一作者：Tao, Fengyun;陶凤云

通讯作者：Zhao, W[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Dept Biomed, Beijing 100023, Peoples R China;[2]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Dept Biomed, 18th Fatou Xili, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2011

卷号：49

期号：5-6

起止页码：369-384

外文期刊名：BIOCHEMICAL GENETICS

收录：;Scopus(收录号：2-s2.0-79958197714);WOS:【SCI-EXPANDED(收录号:WOS:000290537100009)】；

基金：We thank Dr. Jaap J. Beintema and Dr. Helene F. Rosenberg for their valuable suggestions, and Dr. Jinchun Chen for generously providing technical advice. We also thank the anonymous reviewers for their valuable comments. This work was partly supported by funds from the Beijing Education Commission Science and Technology Development Program of China and also by a grant from the Biochemical Engineering College of Beijing Union University.

语种：英文

外文关键词：Amphibian; Antimicrobial activity; Host defense; Positive selection; Ribonuclease

摘要：A novel ribonuclease (RNase) A superfamily gene (Rdronc) has been cloned from the frog Rana dybowskii. The deduced amino acid sequence shows that it belongs to the ribonuclease A superfamily, with the highest identity, 73%, to Rana pipiens onconase. Adaptive evolution analysis based on maximum likelihood models of codon substitution has been conducted on 10 members of the Rana RNases of subcluster B. Rapid adaptive evolution and multiple positive selection sites have been detected, which indicates that these genes may be evolving under positive selection pressure. Functional assay demonstrates that the recombinant Rdronc protein possesses antimicrobial activity against Gram-negative Escherichia coli and Pseudomonas aeruginosa and weaker antimicrobial activity against Gram-positive Staphylococcus aureus and yeast Candida albicans. Our findings support the hypothesis that ribonuclease A superfamily members may function in host defense of early-diversified vertebrates.