文献类型：期刊文献

英文题名：Protective effects of DJ-1 medicated Akt phosphorylation on mitochondrial function are promoted by Da-Bu-Yin-Wan in 1-methyl-4-phenylpyridinium-treated human neuroblastoma SH-SY5Y cells

作者：Zhang, Yi[1,8];Gong, Xiao-Gang[1,9];Wang, Zhen-Zhen[2,3,4];Sun, Hong-Mei[1];Guo, Zhen-Yu[1];Gai, Cong[1];Hu, Jing-Hong[5];Ma, Ling[1];Li, Ping[6];Chen, Nai-Hong[2,3,4,7]

第一作者：Zhang, Yi

通讯作者：Zhang, Y[1];Sun, HM[1]

机构：[1]Beijing Univ Chinese Med, Sch Preclin Med, Dept Anat, 11 North 3rd Ring Eastern Rd, Beijing 100029, Peoples R China;[2]Chinese Acad Med Sci, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China;[3]Chinese Acad Med Sci, Ctr Neurosci, Beijing 100050, Peoples R China;[4]Peking Union Med Coll, Beijing 100050, Peoples R China;[5]Beijing Univ Chinese Med, Sch Preclin Med, Ctr Sci Res, Beijing 100029, Peoples R China;[6]China Japan Friendship Hosp, Inst Clin Med Sci, Beijing Key Lab Immune Mediated Inflammatory Dis, Beijing 100029, Peoples R China;[7]Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China;[8]Harvard Univ, Brigham & Womens Hosp, Sch Med, Ctr Neurol Dis,Dept Neurol, Boston, MA 02115 USA;[9]Beijing Union Univ, Coll Special Educ, Beijing 100075, Peoples R China

第一机构：Beijing Univ Chinese Med, Sch Preclin Med, Dept Anat, 11 North 3rd Ring Eastern Rd, Beijing 100029, Peoples R China

通讯机构：[1]corresponding author), Beijing Univ Chinese Med, Sch Preclin Med, Dept Anat, 11 North 3rd Ring Eastern Rd, Beijing 100029, Peoples R China.

年份：2016

卷号：187

起止页码：83-93

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-84964671203);WOS:【SCI-EXPANDED(收录号:WOS:000378190400010)】；

基金：This study was supported by grants from the National Natural Science Foundation of China (Nos. 81202939, 81473376, 81202507, 81573636, 81573773, and 30873335), Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20120013120007), Beijing Natural Science Foundation (No. 7142115), and Fundamental Scientific Research Funds for Central Public Institute (No. 2014RC03).

语种：英文

外文关键词：Parkinson's disease; Mitochondrial dysfunction; DJ-1; Akt; Phosphorylation; Da-Bu-Yin-Wan

摘要：Ethnopharmacological relevance: Da-Bu-Yin-Wan (DBYW), a historically traditional Chinese medicine formula, was originally defined over 600 years ago. In recent decades, DBYW was clinically employed to treat Parkinson's disease (PD). Aim of the study: To explore the underlying mechanism of DBYW on mitochondrial function, we investigated the effect of DBYW on mitochondrial function from the perspectives of DJ-1 and Akt signaling. Materials and methods: Human derived neuroblastoma SH-SY5Y cells were transiently transfected with the plasmid pcDNA3-Flag-DJ-1 aimed to overexpress the DJ-1 protein. Transfected cells were treated with 1-methyl-4-phenylpyridinium (MPP+), a PD-related mitochondrial complex I inhibitor, in the absence and presence of DBYW. The cell viability was assessed by Cell Counting Kit-8 assay. The protein expressions of DJ-1 and Akt signaling were examined by western blotting. The mitochondrial mass was evaluated by confocal fluorescence microscopy. The mitochondrial complex I activity and cellular ATP content were measured by commercial kits. Results: Transfection with pcDNA3-Flag-DJ-1 decreased the MPP-induced toxicity and overexpressed the DJ-1. In DJ-1 overexpressed cells, the mitochondrial mass was raised, mitochondrial complex I activity was improved, and cellular ATP content was increased. In addition, overexpression of DJ-1 augmented the Akt phosphorylation on threonine 308 and serine 473. Moreover, DBYW promoted the above effects in DJ-1 expressed cells. Conclusions: These data suggest that DJ-1 protects the mitochondria] function by medicating Akt phosphorylation in MPP+-treated SH-SY5Y cells. Moreover, DBYW enhances the protective effect of DJ-1 medicated Akt phosphorylation on mitochondrial function. (C) 2016 Elsevier Ireland Ltd. All rights reserved.