文献类型：期刊文献

中文题名：分光光度法测定附桂骨痛复方中总生物碱的含量

英文题名：Determination of aconitine-type alkaloids in the compound prescription Fugui Gutong by acid dye colorimetry

作者：郑来丽[1,2];田平芳[1];李永生[1,2];乔溪莹[2];王蓉[2];葛喜珍[2]

第一作者：郑来丽

机构：[1]北京化工大学生命科学与技术学院;[2]北京联合大学生物化学工程学院

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2009

期号：4

起止页码：80-83

中文期刊名：北京化工大学学报：自然科学版

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：北京联合大学生化学院生物化工重点建设学科项目(51102630815);北京市教育委员会科技发展计划(KM200611417013)

语种：中文

中文关键词：乌头碱;溴甲酚绿;附桂骨痛

外文关键词：aconitine; bromocresol green; Fugui Gutong

摘要：探讨了附桂骨痛复方中主要有效成分乌头类总生物碱的质量浓度测定方法。依据乌头类总生物碱与溴甲酚绿在pH=3.5的柠檬酸-磷酸氢二钠缓冲液中反应生成离子对化合物的光谱特征,利用分光光度法于412.5nm波长处测定吸收度并计算含量。结果表明,乌头碱含量在10.12～22.77μg/mL范围内与吸收度线性关系良好(r=0.9996,n=6),其平均加样回收率为99.27%。该法用于测定附桂骨痛复方提取液中乌头类总生物碱的含量准确可靠、简便易行、专属性强。
A method for the determination of aconitine-type alkaloids, the main effective components in the compound prescription Fugui Gutong, has been developed. The alkaloid content was determined by acid dye colorimetry, based on the spectrometric features of the ion-pair compounds produced in the reaction between the aconitine-type alkaloids and bromcresol green in pH 3.5 citric acid-disodium hydrogen phosphate buffer solution, with the absorbance being measured at a wavelength of 412.5 nm. The linearity range was 10.12 - 22.77μg/ mL （r = 0. 9996, n =.6）. The average recovery of total alkaloids was 99.27 %. The method is accurate, reliable, and easy to carry out and shows high selectivity for the determation of the content of aconitine-type alkaloids in Fugui Gutong.