文献类型：期刊文献

英文题名：Lipase-catalyzed selective esterification of catechin

作者：Zhou, Juntong[1];Tian, Qingqing[1];Mae, Yuezhu[2];Wang, Yue[1];Huol, Qing[1]

第一作者：Zhou, Juntong

通讯作者：Huol, Q[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Peking Univ, Beijing Yanqing Dist Hosp, Hosp 3, Yanqing Hosp, Beijing 102100, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2021

卷号：11

期号：6

起止页码：995-1000

外文期刊名：MATERIALS EXPRESS

收录：;Scopus(收录号：2-s2.0-85111719446);WOS:【SCI-EXPANDED(收录号:WOS:000665730000023)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Grant Nos. 11475020 and 11975048).

语种：英文

外文关键词：Lipase-Catalyzed; Esterification Reaction of Catechins; Novozym 435; Oil-Soluble

摘要：Catechins show strong antioxidant, antitumoral, antiviral, and anti-inflammatory activities. The uses of catechins in food, cosmetic, and pharmaceutical formulations seem very attractive. Unfortunately, solubility and stability of catechins are poor in apolar media, which limits their efficient uses. In order to improve the solubility of catechins in the oil phase and maintain their oxidation resistance, a regioselective enzymatic acylation was investigated. The effects of reaction medium, water content, carbon chain length of acyl donor and other factors on the acylation reaction were studied, catechins were enzymatically esterified with an aliphatic acid (stearic acid) using an immobilized lipase Novozym 435 in n-butanol. The results show that when the ratio between catechins and stearic acid was 1:5, adding molecular sieves 4A after 11 h of reaction and the temperature of 60 degrees C led to the maximum conversion yield of 60.36%. Studies have shown that catechin stearate has a higher antioxidant activity than vitamin E and dibutyl hydroxytoluene (BHT).