文献类型：期刊文献

英文题名：Combining biosensor and metabolic network optimization strategies for enhanced l-threonine production in Escherichia coli

作者：Zhao, Zhenqiang[1,2]; Zhu, Rongshuai[1,2]; Shi, Xuanping[1,2]; Yang, Fengyu[1,2]; Xu, Meijuan[1,2]; Shao, Minglong[1,2]; Zhang, Rongzhen[1]; Zhao, Youxi[3]; You, Jiajia[1,2]; Rao, Zhiming[1,2]

第一作者：Zhao, Zhenqiang

机构：[1] Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, Wuxi, 214122, China; [2] Institute of Future Food Technology, JITRI, Yixing, 214200, China; [3] College of Biochemical Engineering, Beijing Union University, Beijing, 100023, China

第一机构：Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, Wuxi, 214122, China

年份：2025

卷号：18

期号：1

外文期刊名：Biotechnology for Biofuels and Bioproducts

收录：EI(收录号：20251318128585);Scopus(收录号：2-s2.0-105000969124)

语种：英文

外文关键词：Macroinvertebrates - Mammals - Throughput

摘要：l-threonine is an integral nutrient for mammals, often used in animal feeds to enhance growth and reduce breeding costs. Developing l-threonine engineered strains that meet industrial production specifications has significant economic value. Here, we developed a biosensor that monitors l-threonine concentration to assist in high-throughput screening to capture high-yielding l-threonine mutants. Among them, the PcysK promoter and CysB protein were used to construct a primary l-threonine biosensor, and then the CysBT102A mutant was obtained through directed evolution resulting in a 5.6-fold increase in the fluorescence responsiveness of biosensor over the 0–4?g/L l-threonine concentration range. In addition, the metabolic network of mutant was further optimized through multi-omics analysis and in silico simulation. Ultimately, the THRM13 strain produced 163.2?g/L l-threonine, with a yield of 0.603?g/g glucose in a 5?L bioreactor. The biosensor constructed here could be employed for iterative upgrading of subsequent strains, and these engineering strategies described provide guidance for other chemical overproducers. ? The Author(s) 2025.