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Combining biosensor and metabolic network optimization strategies for enhanced l-threonine production in Escherichia coli  ( EI收录)  

文献类型:期刊文献

英文题名:Combining biosensor and metabolic network optimization strategies for enhanced l-threonine production in Escherichia coli

作者:Zhao, Zhenqiang[1,2]; Zhu, Rongshuai[1,2]; Shi, Xuanping[1,2]; Yang, Fengyu[1,2]; Xu, Meijuan[1,2]; Shao, Minglong[1,2]; Zhang, Rongzhen[1]; Zhao, Youxi[3]; You, Jiajia[1,2]; Rao, Zhiming[1,2]

第一作者:Zhao, Zhenqiang

机构:[1] Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, Wuxi, 214122, China; [2] Institute of Future Food Technology, JITRI, Yixing, 214200, China; [3] College of Biochemical Engineering, Beijing Union University, Beijing, 100023, China

第一机构:Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Jiangsu, Wuxi, 214122, China

年份:2025

卷号:18

期号:1

外文期刊名:Biotechnology for Biofuels and Bioproducts

收录:EI(收录号:20251318128585);Scopus(收录号:2-s2.0-105000969124)

语种:英文

外文关键词:Macroinvertebrates - Mammals - Throughput

摘要:l-threonine is an integral nutrient for mammals, often used in animal feeds to enhance growth and reduce breeding costs. Developing l-threonine engineered strains that meet industrial production specifications has significant economic value. Here, we developed a biosensor that monitors l-threonine concentration to assist in high-throughput screening to capture high-yielding l-threonine mutants. Among them, the PcysK promoter and CysB protein were used to construct a primary l-threonine biosensor, and then the CysBT102A mutant was obtained through directed evolution resulting in a 5.6-fold increase in the fluorescence responsiveness of biosensor over the 0–4?g/L l-threonine concentration range. In addition, the metabolic network of mutant was further optimized through multi-omics analysis and in silico simulation. Ultimately, the THRM13 strain produced 163.2?g/L l-threonine, with a yield of 0.603?g/g glucose in a 5?L bioreactor. The biosensor constructed here could be employed for iterative upgrading of subsequent strains, and these engineering strategies described provide guidance for other chemical overproducers. ? The Author(s) 2025.

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