文献类型：期刊文献

英文题名：Rapid Characterizaiton of Chemical Constituents of the Tubers of Gymnadenia conopsea by UPLC-Orbitrap-MS/MS Analysis

作者：Wang, Xin[1];Zhong, Xiang-Jian[1];Zhou, Na[1];Cai, Ning[1];Xu, Jia-Hui[1];Wang, Qing-Bo[1];Li, Jin-Jie[1];Liu, Qian[1];Lin, Peng-Cheng[2];Shang, Xiao-Ya[1]

第一作者：王欣

通讯作者：Shang, XY[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China;[2]Qinghai Univ Nationalities, Qinghai Prov Key Lab Phytochem Tibetan Plateau, Xining 810000, Peoples R China

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100191, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2020

卷号：25

期号：4

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000522454500134)】；

基金：This work was supported by the grants from the Scientific Research Common Program of Beijing Municipal Commission of Education (Grant No. KM201911417014 and KM201811417003), Premium Funding Project for Academic Human Resources Development in Beijing Union University (BPHR2019DZ02), and Key projects of the Beijing Natural Sciences Foundation and Beijing Municipal Education Committee (No. KZ201811417049).

语种：英文

外文关键词：Gymnadenia conopsea; UPLC-Orbitrap-MS/MS; chemical constituents; rapid characterization

摘要：Gymnadenia conopsea R. Br. is a traditional Tibetan medicinal plant that grows at altitudes above 3000 m, which is used to treat neurasthenia, asthma, coughs, and chronic hepatitis. However, a comprehensive configuration of the chemical profile of this plant has not been reported because of the complexity of its chemical constituents. In this study, a rapid and precise method based on ultra-high performance liquid chromatography (UPLC) combined with an Orbitrap mass spectrometer (UPLC-Orbitrap-MS/MS) was established in both positive- and negative-ion modes to rapidly identify various chemical components in the tubers of G. conopsea for the first time. Finally, a total of 91 compounds, including 17 succinic acid ester glycosides, 9 stilbenes, 6 phenanthrenes, 19 alkaloids, 11 terpenoids and steroids, 20 phenolic acid derivatives, and 9 others, were identified in the tubers of G. conopsea based on the accurate mass within 3 ppm error. Furthermore, many alkaloids, phenolic acid derivates, and terpenes were reported from G. conopsea for the first time. This rapid method provides an important scientific basis for further study on the cultivation, clinical application, and functional food of G. conopsea.