文献类型：期刊文献

中文题名：吡咯喹啉醌生产菌的发酵条件优化

英文题名：Optimization of fermentation conditions for pyrroloquinoline quinone expression by Pseudomonas 0813

作者：钟杉杉[1];刘辉[2];葛喜珍[3];田平芳[1]

第一作者：钟杉杉

机构：[1]北京化工大学生命科学与技术学院;[2]北京天坛生物制品股份有限公司;[3]北京联合大学生物化学工程学院

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2013

卷号：40

期号：5

起止页码：88-92

中文期刊名：北京化工大学学报：自然科学版

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金(21076013/21276014)

语种：中文

中文关键词：吡咯喹啉醌;发酵;单因素试验;正交试验

外文关键词：pyrroloquinoline quinone ; fermentation; single factor test ; orthogonal test

摘要：对一株产吡咯喹啉醌(PQQ)假单胞杆菌Pseudomonas 0813的发酵条件进行了优化,通过单因素试验确定碳源、氮源及无机盐成分,之后用正交试验法优化各成分配比,考察了发酵温度、初始pH值和转速对该菌产PQQ的影响。结果表明,最优的培养基配方为:酵母粉5 g/mL、蛋白胨1 g/mL、KH2PO40.5 g/mL;最适的发酵条件为:发酵温度30℃、初始pH 6.5、发酵转速150 r/min。在此条件下,Pseudomonas 0813菌株在250 mL体系摇瓶发酵中的PQQ产量为448 mg/L,在1.5 L体系发酵罐扩大培养后的最高产量可达695 mg/L,这是目前报道未经优化或基因改造菌株的较高产量。
A pyrroloquinoline quinone （PQQ）-synthesizing stain Pseudomonas 0813 has been fermented and the fermentation conditions were optimized. Single factor tests were carried out to determine the appropriate carbon source, nitrogen source and inorganic salt. Orthogonal tests were performed to determine the optimum ratios of the components in the fermentation medium. The cultivation temperature, initial pH value and rotation speed were also optimized. The results showed that the optimum medium composition was yeast extract 5 g/mL, tryptone 1 g/mL, and KH2PO4 0.5 g/mL, whilst the optimized fermentation conditions were a fermentation temperature of 30 ℃, an initial pH value of 6.5 and a rotation speed of 150r/min. Using the above conditions, the yields of PQQ using this strain reached 448 mg/L and 695 mg/L in a 250 mL shaken flask and a 1.5 L fermenter, respectively. These are notable yields for wild type strains devoid of mutagenesis and genetic modification.