文献类型：期刊文献

英文题名：Natural compound berberine inhibits apple ring rot fungus Botryosphaeria dothidea

作者：Li, Pengyu[1];Liu, Jinrong[2];Li, Ying[1];Yang, Tao[1];Ge, Xizhen[1];Tian, Pingfang[2]

第一作者：Li, Pengyu

通讯作者：Ge, XZ[1];Tian, PF[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：0

外文期刊名：INTERNATIONAL JOURNAL OF PEST MANAGEMENT

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000772965200001)】；

基金：This work was supported by grants from Project of Beijing Municipal Commission of Education (KZ201911417049), and Key Research and Development Program of Hebei province of China (19226509 D, 20322501 D).

语种：英文

外文关键词：Apple ring rot; berberine; Botryosphaeria dothidea; enzyme activity; inhibition rate

摘要：Apple ring rot is a disease caused by Botryosphaeria dothidea that severely reduces apple yield. Currently, management of this disease relies primarily on chemical fungicides. However, chemical fungicides cause fruit contamination. Here we report that berberine, an alkaloid extracted from medicinal plant Coptis chinensis, can efficiently inhibit the B. dothidea isolates collected from different regions in China. The 50% effective concentration (EC50) and minimum inhibitory concentration (MIC) of berberine for B. dothidea were 8.4 similar to 9.8 mu g mL(-1) and 17.1 similar to 20 mu g mL(-1), respectively. Scanning electronic microscope showed efficient inhibition of berberine against the hyphal growth of B. dothidea. Conductivity analysis revealed that berberine increased the membrane permeability of B. dothidea. Remarkably, berberine repressed the activities of succinate dehydrogenase and malate dehydrogenase, two enzymes participating in energy metabolism. In vitro experiments revealed strong inhibition of berberine against the B. dothidea on detached fruits. Field trials showed 78.27% inhibition rate of berberine toward B. dothidea. Collectively, berberine is a promising natural fungicide to control apple ring rot because of its high efficacy, low cost, and safety to human health.