文献类型：期刊文献

中文题名：生物合成薯蓣皂素的途径设计及关键酶分析

英文题名：Pathway design and key enzyme analysis of diosgenin biosynthesis

作者：孙忠义[1];赵鹏[1];葛喜珍[2];田平芳[1]

第一作者：孙忠义

机构：[1]北京化工大学生命科学与技术学院,北京100029;[2]北京联合大学生物化学工程学院,北京100023

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2021

卷号：37

期号：4

起止页码：1178-1188

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；PubMed;

基金：国家重点研发计划(No.2018YFA0901800);河北省重点研发计划(Nos.20322501D,19226509D);北京市教育委员会科研计划-北京市自然科学基金项目(No.KZ201911417049)资助。

语种：中文

中文关键词：薯蓣皂素;生物合成途径;关键酶;异源表达

外文关键词：diosgenin;biosynthetic pathway;key enzymes;heterologous expression

摘要：薯蓣皂素是一种天然甾体皂苷元,可作为数百种类固醇药物的前体,具有重要药用价值。目前工业生产薯蓣皂素主要依赖化学提取法,因此该法依赖植物材料和耕地且对环境有害。随着代谢工程和合成生物学的发展,生物合成法受到广泛关注。文中综述了生物合成薯蓣皂素的代谢途径和关键酶,并在酿酒酵母Saccharomyces cerevisiae中设计其异源合成途径,提出改造策略,以期为全生物合成薯蓣皂素提供有价值的参考。
As a naturally occurring steroid sapogenin,diosgenin acts as the precursor of hundreds of steroid medicines,and thereby has important medicinal value.Currently,industrial production of diosgenin relies primarily on chemical extraction from plant materials.Clearly,this strategy shows drawbacks of excessive reliance on plant materials and farmland as well as environment pollution.Due to development of metabolic engineering and synthetic biology,bio-production of diosgenin has garnered plenty of attention.Although the biosynthetic pathways of diosgenin have not been completely identified,in this review,we outline the identified biosynthetic pathways and key enzymes.In particular,we suggest heterologous biosynthesis of diosgenin in Saccharomyces cerevisiae.Overall,this review aims to provide valuable insights for future complete biosynthesis of diosgenin.