文献类型：期刊文献

英文题名：3-Hydroxypropionaldehyde-specific aldehyde dehydrogenase from Bacillus subtilis catalyzes 3-hydroxypropionic acid production in Klebsiella pneumoniae

作者：Su, Mingyue[1]; Li, Ying[1]; Ge, Xizhen[2]; Tian, Pingfang[1]

第一作者：Su, Mingyue

通讯作者：Tian, Pingfang

机构：[1] Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China; [2] College of Biochemical Engineering, Beijing Union University, Beijing, 100023, China

第一机构：Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

年份：2015

卷号：37

期号：3

起止页码：717-724

外文期刊名：Biotechnology Letters

收录：EI(收录号：20144900286831);Scopus(收录号：2-s2.0-84925489920)

语种：英文

外文关键词：Bacteria - Bacteriology - Catalyst activity - Aldehydes - Substrates

摘要：In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we report that DhaS, a putative ALDH from Bacillus subtilis, shows high specificity toward 3-HPA when heterologously expressed in K. pneumoniae. Using NAD+ as a cofactor, DhaS exhibited higher catalytic activity (2.3 U mg?1) and lower Km value (0.4?mmol?l?1) toward 3-HPA than that toward other aldehydes. Under shake-flask conditions, the recombinant strain produced 2.1?g 3-HP l?1 in 24?h, which is 3.9-fold of that in a control harboring a blank vector. Under non-optimized bioreactor conditions, the recombinant strain produced 18?g 3-HP l?1 and 1,3-propanediol (1,3-PDO) at 27?g?l?1 in 24?h. The overall conversion rate from glycerol to 3-HP and 1,3-PDO reached 59.4?mol?mol?1. Homology modeling of DhaS illustrates substrate specificity and NAD+-binding site. DhaS is thus a 3-HPA-specific enzyme useful for production of 3-HP. ? 2014, Springer Science+Business Media Dordrecht.