文献类型：期刊文献

英文题名：Isolation and Expression of a New High Molecular Weight Glutenin Subunit Gene at the Glu-D-1-2 Locus from Aegilops tauschii

作者：Zhang, Y.[1,2];An, X.[1];Li, X.[1];Chen, S.[1];Gao, L.[1];Wang, K.[1];Wang, S.[1];Yan, Y.[1]

第一作者：Zhang, Y.;张瀛

通讯作者：Yan, Y[1]

机构：[1]Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China;[2]Beijing Union Univ, Coll Appl Sci & Humanities, Beijing 100083, Peoples R China

第一机构：Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China

通讯机构：[1]corresponding author), Capital Normal Univ, Coll Life Sci, Key Lab Genet & Biotechnol, Beijing 100048, Peoples R China.

年份：2009

卷号：37

期号：3

起止页码：449-457

外文期刊名：CEREAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000269638100014)】；

基金：This research was financially supported by grants from the National Natural Science Foundation of China ( 30771334, 30830072), the Ministry of Science and Technology of China (2009CB118303, 2006AA10Z186) and the Key Project of Natural Science Foundation of Beijing (KZ200910028003).

语种：英文

外文关键词：Aegilops tauschii; common wheat; HMW-GS; SDS-PAGE; MALDI-TOF-MS; protein post-translational modifications

摘要：Two new y-type HMW-GSs in Ae. tauschii, 1Dy12.1*(t) and 1Dy12.2(t) with the mobility order of 1Dy12.2(t) > 1Dy12.1*(t) > 1Dy12.1(t) > 1Dy12, were identified by both SDS-PAGE and MALDI-TOF-MS. Molecular cloning and sequencing showed that the genes encoding sub-units 1Dy12.1*(t) and 1Dy12.2(t) had identical nucleotide acid sequences with 1,947 bp encoding a mature protein of 627 residues. Their deduced molecular weights were 67,347.6 Da, satisfactorily corresponding to that of 1Dy12.2(t) subunit determined by MALDI-TOF-MS (67,015.7 Da), but was significantly smaller than that of the the 1Dy12.1*(t) subunit (68,577.1 Da). Both subunits showed high similarities to 1Dy10, suggesting that they could have a positive effect on bread-making quality. Interestingly, the expressed protein of the cloned ORF from accessions TD87 and TD130 in E. coli co-migrated with subunit 1Dy12.2(t), but moved slightly faster than 1Dy12.1*(t) on SDS-PAGE. The expressed protein in transgenic tobacco seeds, however, had the same mobility as the 1Dy12.1*(t) subunit, as confirmed by both SDS-PAGE and Western blotting. Although direct evidence of phosphoprotein could not be obtained by specific staining method, certain types of post-translational modifications (PTMs) of the 1Dy12.1*(t) subunit could not be excluded. We believe PTMs might be responsible for the molecular weight difference between the subunits 1Dy12.1*(t) and 1Dy12.2(t).