文献类型：期刊文献

中文题名：高产碱性果胶酶菌株的育种及其发酵条件的研究

英文题名：Breeding of high producing strain of alkaline pectinase and its solid-state fermentation conditions

作者：李祖明[1];李鸿玉[1];何立千[1];白志辉[2];荣瑞芬[1];全菲[1];叶磊[1];李丽云[1];李京霞[1];厉重先[1]

机构：[1]北京联合大学师范学院;[2]中科院生态环境研究中心

第一机构：北京联合大学师范学院

年份：2008

卷号：38

期号：3

起止页码：27-32

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：北京市优秀人才培养资助项目"碱性果胶解聚酶植物抗病剂的研制"(20061D0502200295)

语种：中文

中文关键词：碱性果胶酶;紫外诱变;固态发酵;酶学性质

外文关键词：alkaline pectinase; UV mutagenesis; solid-state fermentation; enzyme properties

摘要：以克劳氏芽孢杆菌（Bacillus clausii）S4为出发菌株，经紫外诱变育种和固态发酵条件的选育，得到产碱性果胶酶较高的新菌株N-10，并研究了其固态发酵条件和部分酶学性质。结果表明，以甜菜渣为碳源和酶的诱导物以及棉粕作为氮源较适宜。较优的固态发酵条件为：甜菜渣5g，棉粕0．125g，麦芽糖0．1g，KH2PO4 0．0075g，Na2CO3 0．15g，水12．5mL，种龄24h，接种量2mL，发酵温度40℃，发酵时间84h；酶产率可达4780u／g（干甜菜渣），较菌株S-4提高108％。该酶的最适pH10，最适温度55℃；分别在pH7．5～9．5和30--40℃范围内较稳定；Ca^2＋、Mg^2＋、Fe^2＋对酶活有明显激活作用，Cu2^2＋、Zn^2＋对酶活有强烈抑制活作用。
A high yielding strain Bacillus dausii N-10 was obtained by UV mutagenesis from Bacillus clausii S-4 for the production of alkaline pectinase. Solid state fermentation（SSF） conditions and partial properties of alkaline pectinase for the mutant strain N-10 were investigated. Sugar beet pulp was a good enzyme inducer and carbon source, while cottonseed meal was a good nitrogen source. After inoculated with 2 mL of a 24-h-old inoculum culture and incubated at 40℃ for 84 h by SSF, the maximum alkaline pectinase yield of 4780 U/g dry sugar beet pulp from BaciLLus clausii N-10 was obtained in the medium contained 5.0 g dry sugar beet pulp, 0. 125 g cottonseed meal, 0.1 g maltose, 0. 0075 g KH2PO4, 0.15 g Na2CO3 and 12.5 mL water, which was increased by 108% if compared with that of its initial strain S-4. The highest activity of the crude enzyme from BaciLLus clausii N-10 was at pH 10 and 55 C, respectively. The enzyme was stable from pH 7.5 to pH 9.5 at 40 C for 30 min. The activity of the enzyme was enhanced by Ca^2＋ , Mg^3＋ and Fe^2＋ ions, whereas was inhibited by Cu^2＋ and Zn^2＋ ions.