文献类型：期刊文献

英文题名：Developing IR-780 as a Novel Matrix for Enhanced MALDI MS Imaging of Endogenous High-Molecular-Weight Lipids in Brain Tissues

作者：Li, Na[1,3];Wang, Peng[2];Liu, Xiaolong[1];Han, Chao[1,3];Ren, Wei[1,3];Li, Tuo[1,3];Li, Xing[1,3];Tao, Fengyun[2];Zhao, Zhenwen[1,3]

第一作者：Li, Na

通讯作者：Zhao, ZW[1];Zhao, ZW[2]

机构：[1]Chinese Acad Sci, Inst Chem,Key Lab Mol Nanostruct & Nanotechnol, Beijing Mass Spectrum Ctr,Key Lab Analyt Chem Liv, CAS Res Educ Ctr Excellence Mol Sci,Beijing Natl, Beijing 100190, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[3]Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

第一机构：Chinese Acad Sci, Inst Chem,Key Lab Mol Nanostruct & Nanotechnol, Beijing Mass Spectrum Ctr,Key Lab Analyt Chem Liv, CAS Res Educ Ctr Excellence Mol Sci,Beijing Natl, Beijing 100190, Peoples R China

通讯机构：[1]corresponding author), Chinese Acad Sci, Inst Chem,Key Lab Mol Nanostruct & Nanotechnol, Beijing Mass Spectrum Ctr,Key Lab Analyt Chem Liv, CAS Res Educ Ctr Excellence Mol Sci,Beijing Natl, Beijing 100190, Peoples R China;[2]corresponding author), Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.

年份：2019

卷号：91

期号：24

起止页码：15873-15882

外文期刊名：ANALYTICAL CHEMISTRY

收录：;EI(收录号：20195107863229);Scopus(收录号：2-s2.0-85076389968);WOS:【SCI-EXPANDED(收录号:WOS:000503910600073)】；

基金：This work was supported by the National Key R&D Program of China (Grant Nos. 2018YFA0506900), and National Natural Science Foundation of China (21575146, 21635008, and 21621062). We are grateful to Prof. Jiang Zhou (Peking University, Beijing, People's Republic of China) for his technical assistance in the detection of CCS.

语种：英文

外文关键词：Brain - Desorption - Histology - Mass spectrometry - Molecular weight - Optical properties - Phospholipids - Tissue

摘要：The matrix plays a prominent role in expanding the ability of matrix assisted laser desorption/ionization mass spectrometry (MALDI MS). However, on account of the unclarity of necessary properties of the matrix in MALDI MS, development of a new matrix is still in the exploratory stage and lacks systematic theoretical guidance. Meanwhile, most of the existing matrices are unable to simultaneously detect various high-molecular-weight (high-MW) lipids including (poly-)phosphoinositides, cardiolipins, and gangliosides. In this study, we have successfully screened and optimized the application of commercially available IR-780 as a novel matrix for simultaneously profiling and imaging high-MW lipids in brain tissues by MALDI MS for the first time. The properties of IR-780 related to the matrix of MALDI MS, mainly including the optical properties (UV absorption, fluorescence emission, and photothermal efficiency), proton affinity, collision cross-sections (CCSs), salt-tolerance ability, and homogeneity, were comprehensively characterized, which demonstrated that high photothermal ability and large CCSs might guarantee the superior performance of IR-780 as matrix for the analysis of high-MW lipids in biological samples. This work provided some references for the development of a novel matrix, and especially, the concept of CCS was first introduced as a parameter for the development of a matrix. In addition, the simultaneous identification and imaging of endogenous high-MW lipids in rat brain tissues subjected to traumatic brain injury were successfully performed.