文献类型：期刊文献

英文题名：Saprophytic Bacillus Accelerates the Release of Effective Components in Agarwood by Degrading Cellulose

作者：Yang, Huizhen[1];He, Runying[1];Cui, Yao[1];Li, Ying[1];Ge, Xizhen[1]

通讯作者：Li, Y[1];Ge, XZ[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2022

卷号：27

期号：4

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000764314200001)】；

基金：FundingThis work was financially supported by Project of Beijing Municipal Commission of Education (KM202011417006); Beijing Municipal Education Commission Technology Plan (KZ201911417049).

语种：英文

外文关键词：saprophytic Bacillus; Agarwood; cellulose; effective components

摘要：The value of Agarwood increases with time due to the gradual release of its major components, but the mechanism behind this remains unclear. Herein we reveal that the potential driving force of this process is the degradation of cellulose in Agarwood by its saprophytic Bacillus subtilis. We selected 10-year-old Agarwood from different places and then isolated the saprophytic bacteria. We confirmed these bacteria from different sources are all Bacillus and confirmed they can degrade cellulose, and the highest cellulase activity reached 0.22 U/mL. By co-cultivation of the bacterium and Agarwood powder, we found that three of the strains could release the effective components of Agarwood, while they had little effect in increasing the same components in living Aquilaria sinensis. Finally, we demonstrated that these saprophytic Bacillus subtilis have similar effects on Zanthoxylum bungeanum Maxim and Dalbergiaod orifera T. Chen, but not on Illicium verum Hook. f, Cinnamomum cassia Presl and Phellodendron chinense Schneid. In conclusion, our experiment revealed that the saprophytic Bacillus release the effective components of Agarwood by degrading cellulose, and we provide a promising way to accelerate this process by using this bacterial agent.