文献类型：期刊文献

英文题名：Study on titanium dioxide nanoparticles as MALDI MS matrix for the determination of lipids in the brain

作者：Peng, Congnan[1];Zhang, Qian[1];Liu, Jian-an[2];Wang, Zhen-peng[2];Zhao, Zhen-wen[2];Kang, Ning[1];Chen, Yuxin[1];Huo, Qing[1]

第一作者：Peng, Congnan

通讯作者：Huo, Q[1]

机构：[1]Beijing Union Univ, Dept Biomed, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Chinese Acad Sci, Beijing Mass Spectrum Ctr, Inst Chem, Beijing 100190, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Dept Biomed, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2021

卷号：10

期号：1

起止页码：700-710

外文期刊名：GREEN PROCESSING AND SYNTHESIS

收录：;EI(收录号：20224413048435);Scopus(收录号：2-s2.0-85120384353);WOS:【SCI-EXPANDED(收录号:WOS:000718184600001)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Grants No. 11475020 and 11975048) and Beijing Union University Graduate Program.

语种：英文

外文关键词：TiO2 NPs; MALDI mass spectrometry; lipid

摘要：The structures of lipids are diverse, and thus, lipids show various biological functions. Systematic determination of lipids in organisms has always been a concern. In this paper, a methodology on the matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), with titanium dioxide nanoparticles (TiO2 NPs) as the matrix, was studied for lipid determination. The results showed that the following conditions were preferable in the determination of small-molecule lipids (such as hypoxanthine, guanosine, uridine, and cytidine), lipid standards (such as GC, GM, TG, phosphatidylethanolamine, phosphatidylcholine, and ceramide), and mixed lipids (extracted from brain homogenate with methanol alone and with the B&D method): TiO2 NPs as the matrix, absolute ethanol as the solvent, 1 mg of TiO2 NPs dispersed in 1 mL of absolute ethanol as the matrix solution, NaCl as the ionization reagent, and positive mass spectrometry (MS) as the mode. Modified TiO2 NP as a new matrix for MALDI MS will be a future research direction; in addition, the characteristics of TiO2 NPs make it a potential matrix for imaging MS.