文献类型：期刊文献

英文题名：Anti-Tumor Effects of Astaxanthin by Inhibition of the Expression of STAT3 in Prostate Cancer

作者：Sun, Shao-Qian[1];Zhao, You-Xi[1];Li, Si-Yu[1];Qiang, Jing-Wen[1];Ji, Yi-Zhi[1]

第一作者：孙少倩

通讯作者：Ji, YZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2020

卷号：18

期号：8

外文期刊名：MARINE DRUGS

收录：;Scopus(收录号：2-s2.0-85089407787);WOS:【SCI-EXPANDED(收录号:WOS:000564061800001)】；

基金：This work was supported by grants from Scientific Research Common Program of Beijing Municipal Commission of Education (No. KM201911417013) and Premium Funding Project for Academic Human Resources Development in Beijing Union University (No. BPHR2019DZ04).

语种：英文

外文关键词：prostate cancer; astaxanthin; STAT3; proliferation; colony formation; apoptosis; migration; invasion

摘要：Astaxanthin is a natural product gaining increasing attention due to its safety and anti-cancer properties. In this study, we investigated the mechanisms of the anti-cancer effects of astaxanthin on prostate cancer (PCa) cell lines using aggressive PCa DU145 cells. Also an instantaneous silenced cell line (si-STAT3) derived from DU145 and a control cell line (si-NK) were used for the MTT and colony formation assays to determine the role of astaxanthin in proliferation and colony formation abilities. Flow cytometry assays were used to detect the apoptosis of tumor cells. Migration and invasion assays detected the weakening of the respective abilities. Western blot and RT-PCR tests detected the levels of STAT3 protein and mRNA. Astaxanthin resulted in suppression of the proliferation of DU145 cells and the level of STAT3. The treatment of DU145 cells with astaxanthin decreased the cloning ability, increased the apoptosis percentage and weakened the abilities of migration and invasion of the cells. Furthermore, astaxanthin reduced the expression of STAT3 at protein and mRNA levels. The effects were enhanced when astaxanthin and si-STAT3 were combined. The results of animal experiments were consistent with the results in cells. Thus, astaxanthin inhibits the proliferation of DU145 cells by reducing the expression of STAT3.