文献类型：期刊文献

中文题名：微生物法测定婴幼儿配方食品中生物素的不确定度评估

英文题名：Uncertainty Evaluation for Microbiological Assay of Biotin in Formula

作者：商迎辉[1];章丽娜[1];李梦洁[1];赵范范[1];刘馨君[1];魏俊[1];黄汉昌[1];劳凤学[1]

第一作者：商迎辉

机构：[1]北京市生物活性物质与功能食品研究院北京联合大学功能因子与脑科学研究院生物化学工程学院

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

年份：2019

卷号：19

期号：10

起止页码：221-226

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute of Food Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金面上项目(31471587)

语种：中文

中文关键词：微生物法;婴幼儿配方食品;生物素;不确定度

外文关键词：microbiological assay;formula;biotin;uncertainty

摘要：按照食品安全国家标准GB 5009.259-2016规定的微生物法对婴幼儿配方食品中生物素含量进行测定,根据CNAS-CL07:2011要求并依据JJF1059.1-2012和GB/Z22553-2010对测定过程影响检验结果的各个分量进行评估,即对测量重复性,试样称量及稀释,标准溶液配制,标准曲线拟合及紫外-可见光分光光度计测量偏差5个方面引入的不确定度进行分析,并确定了各不确定度分量,得出扩展不确定度。经测定,婴幼儿谷粉中生物素含量为(32.49±2.64)μg/100 g,P=95%,k=2。微生物因素对测量不确定度影响显著,由标准曲线拟合和重复测量体现。
Based on CNAS-CL07:2011,the uncertainty for biotin microbiological assay in formula refer to GB 5009.259-2016 should be evaluated.According to JJF1059.1-2012 and GB/Z22553-2010,the sources of uncertainty for the method were explored.The evaluation involves repeatability of measurement,weighing of sample and sample dilution,standard solution preparation,standard curve fitting and measured deviation of UV-Visible spectrophotometer.By analysis,the heft of uncertainty and expanded uncertainty were calculated.The content of biotin in cereal meal for infant was(32.49±2.64)μg/100 g,P=95%,k=2.The most significant factor that contributed to the determination of biotin was microbiological factors,they were shown in standard curve fitting and repeatability of measurement.