文献类型：期刊文献

英文题名：Effect of magnesium ions/Type i collagen promote the biological behavior of osteoblasts and its mechanism

作者：Nie, Xiaojing[1]; Sun, Xirao[1]; Wang, Chengyue[1]; Yang, Jingxin[2]

第一作者：Nie, Xiaojing

通讯作者：Wang, Chengyue

机构：[1] Department of Prosthodontics, Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China; [2] College of Robotics, Beijing Union University, Beijing, 100000, China

第一机构：Department of Prosthodontics, Second Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China

年份：2019

卷号：7

期号：1

起止页码：53-61

外文期刊名：Regenerative Biomaterials

收录：EI(收录号：20201308342166);Scopus(收录号：2-s2.0-85082086040)

语种：英文

外文关键词：Biocompatibility - Cell signaling - Coatings - Collagen - Magnesium - Metal ions - Phosphatases - Rhodamine B

摘要：Type I collagen (Col I) is a main component of extracellular matrix (ECM). Its safety, biocompatibility, hydrophilicity and pyrogen immunogenicity make it suitable for tissues engineering applications. Mg2+ also control a myriad of cellular processes, including the bone development by enhancing the attachment and differentiation of osteoblasts and accelerating mineralization to enhance bone healing. In our studies, Mg2+ bind collagen to promote the proliferation and differentiation of osteoblasts through the expression of integrins and downstream signaling pathways. In order to clarify the biological behavior effect of 10 mM Mg2+/Col I coating, we performed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase (ALP), 4′6-diamidino-2-phenylindole (DAPI), Alizarin red staining and Rhodamine B-isothiocyanate (RITC)-labeled phalloidin experiments and found that 10 mM Mg2+ group, Col I-coating group, 10 mM Mg2+/Col I-coating group, respectively, promoted the proliferation and differentiation of osteoblasts, especially 10 mM Mg2+/Col I-coating group. We detected the mRNA expression of osteogenic-related genes (Runx2, ALP and OCN, OPN and BMP-2) and the protein expression of signaling pathway (integrin α2, integrin β1, FAK and ERK1/2), these results indicated that 10 mM Mg2+/Col I coating play an critical role in up-regulating the MC3T3-E1 cells activity. The potential mechanisms of this specific performance may be through activating via integrin α2β1-FAK-ERK1/2 protein-coupled receptor pathway. ? 2019 The Author(s) 2019. Published by Oxford University Press.