文献类型：期刊文献

英文题名：Lactobacillales derived from traditional Xizang dairy products improve insomnia and restore neurotransmitter-metabolic profiles via gut microbiota in PCPA-induced mice

作者：Li, Peifan[1,4];Yang, Ling[2];Shao, Xinyu[1];Zou, Zhi[3];Shi, Hanyu[1];Sun, Yongbing[3];Wu, Xiaoling[3];Li, Zhonglin[3];Li, Yongli[3];Li, Zuming[1]

第一作者：Li, Peifan

通讯作者：Li, ZM[1]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Hebei Inatural Biotech Co Ltd, Shijiazhuang 050800, Peoples R China;[3]Zhengzhou Univ, Henan Prov Peoples Hosp, Peoples Hosp, Zhengzhou 450003, Peoples R China;[4]Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2025

卷号：300

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20252818761295);Scopus(收录号：2-s2.0-105010194215);WOS:【SCI-EXPANDED(收录号:WOS:001537432400001)】；

基金：This work was supported by the Henan Province Key Research and Development Special Fund (251111312900) ; Central Plains Science and Technology Innovation Leading Talent Program Fund (244200510016) ; Henan Province Science and Technology Key Research and Development Program Project Fund (252102310050) ; Henan Province Medical Science and Technology Key Project Fund (LHGJ20240050, SBGJ202402100) , Hebei Inatural Biotech Co. Ltd. Cooperation Projects Fund (50304JA2306) and "Qimingxing" Science and Technology Inno-vation Project Fund (20051061) .

语种：英文

外文关键词：Lactobacillales; Insomnia; Gut microbiota; Metabolomics; Neurotransmitters

摘要：Insomnia remains a major public health challenge with limited safe treatment options. We demonstrate that mixed probiotics Lactobacillales (Lactobacillus helveticus L551, Lacticaseibacillus paracasei L578, and Streptococcus thermophilus S709, group P) alleviates insomnia in PCPA-induced mice. P significantly reduced sleep latency and increased sleep duration, while ameliorating anxiety behaviors. The intervention attenuated insomnia-induced colonic structural damage and reactive oxygen species (ROS) accumulation, while restoring hippocampal CA1sp and hypothalamic Nissl body density, as well as normalizing brain glucose metabolism. Crucially, P intervention rebalanced neurotransmitter networks by enhancing serotonin (5-HT) synthesis while suppressing its catabolism, and reducing dopamine (DA) and norepinephrine (NE) levels. The P intervention significantly increased the abundance of Akkermansia and Acinetobacter compared to the insomnia model group (M). Notably, Acinetobacter exhibited significant positive correlations with N-acetylserotonin and gamma-aminobutyric acid (GABA) levels, while showing a significant negative correlation with 5-hydroxyindoleacetic acid (5-HIAA). These findings suggest its potential capacity to modulate tryptophan metabolism. In the M group, Staphylococcus was identified as the signature taxon, showing significant positive correlations with sleep latency and DA levels, and significant negative correlations with sleep duration and serotonin 5-HT levels. P intervention significantly reduced Staphylococcus abundance, concomitant with DA level normalization. In summary, Lactobacillales improve sleep quality and relieve anxiety in insomnia mice via modulating gut microbiota composition, thus regulating tryptophan metabolic pathways and sleep - related neurotransmitters.