文献类型：期刊文献

英文题名：Determination of 4 psychoactive substances in tea using ultra high performance liquid chromatography combined with the quadrupole time-of-flight mass spectrometry

作者：Li, Jian[1,3];Ma, Junmei[2,4];Zhang, Yan[2,4];Zheng, Lei[1]

第一作者：李健;Li, Jian

通讯作者：Zheng, L[1];Zhang, Y[2];Zhang, Y[3]

机构：[1]Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China;[2]Hebei Med Univ, Coll Forens Med, Hebei Key Lab Forens Med, Shijiazhuang 050017, Peoples R China;[3]Beijing Union Univ, Coll Appl Arts & Sci, Beijing 100191, Peoples R China;[4]Hebei Food Inspect & Res Inst, Hebei Food Safety Key Lab, Shijiazhuang 050227, Peoples R China

第一机构：Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China

通讯机构：[1]corresponding author), Hefei Univ Technol, Sch Food & Biol Engn, Hefei 230009, Peoples R China;[2]corresponding author), Hebei Med Univ, Coll Forens Med, Hebei Key Lab Forens Med, Shijiazhuang 050017, Peoples R China;[3]corresponding author), Hebei Food Inspect & Res Inst, Hebei Food Safety Key Lab, Shijiazhuang 050227, Peoples R China.

年份：2020

卷号：12

期号：40

起止页码：4878-4884

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20204509454293);Scopus(收录号：2-s2.0-85094221994);WOS:【SCI-EXPANDED(收录号:WOS:000580651100004)】；

基金：This work was supported by National Key Research and Development Program of China (Project No. 2018YFC1603400).

语种：英文

外文关键词：High performance liquid chromatography - Tea

摘要：In this study, a method for the qualification and quantification of 4 psychoactive substances in tea using ultra high performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) has been developed. Tea samples were extracted by a 50% (v/v) methanol-water solution, and then separated by an ACQUITY UPLC BEH Shield RP18 column using a binary solvent system by gradient elution. The analytes were determined by Q-TOF/MS in TOFMS and information-dependent acquisition (IDA)-MS/MS mode. The results showed that the mass accuracy error of the 4 psychoactive substances were lower than 5.0 x 10(-6), and a good linear relationship was observed in the range of 0.5-500 mu g L(-1)and correlation coefficient was higher than 0.9990. The LOD was in the range of 0.005-0.020 mg kg(-1)and the LOQ was in the range of 0.010-0.040 mg kg(-1). The recovery of the method was in range of 80.14-93.25% with spike levels of 0.010-0.400 mg kg(-1), and relative standard deviations were lower than 10%. The method was simple, specific and reliable. It has been successfully used for the detection of 4 psychoactive substances in tea samples.