文献类型：期刊文献

英文题名：Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Haloarchaeon Halogranum amylolyticum

作者：Zhao, You-Xi[1,2]; Rao, Zhi-Ming[1]; Xue, Yan-Fen[3]; Gong, Ping[2]; Ji, Yi-Zhi[2]; Ma, Yan-He[3]

第一作者：赵有玺;Zhao, You-Xi

通讯作者：Rao, Zhi-Ming

机构：[1] The Key Lab of Industrial Biotechnology, the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; [2] Biochemical Engineering College, Beijing Union University, Beijing, 100023, China; [3] State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

第一机构：The Key Lab of Industrial Biotechnology, the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

通讯机构：[1]The Key Lab of Industrial Biotechnology, the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

年份：2015

卷号：99

期号：18

起止页码：7639-7649

外文期刊名：Applied Microbiology and Biotechnology

收录：EI(收录号：20151900833495);Scopus(收录号：2-s2.0-84939573609)

语种：英文

外文关键词：Fermentation - Nuclear magnetic resonance - Optimization - Bacteria - Carbon - Mass spectrometry - Polymerase chain reaction - Cloning - Gas chromatography - Glucose - Oligonucleotides

摘要：Haloarchaea is an important group of polyhydroxyalkanoate (PHA)-accumulating organisms. However, few promising haloarchaeal species for economical and efficient PHA production have been reported. Here, we first discovered that Halogranum amylolyticum TNN58 could efficiently accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a high 3-hydroxyvalerate (3HV) fraction using glucose as carbon source. Briefly, transmission electron microscopy (TEM) analysis revealed the presence of a large number of PHA granules in the cells. Gas chromatography–mass spectrometry (GC-MS) and proton nuclear magnetic resonance (1H NMR) analyses showed that PHAs synthesized from glucose was PHBV. Moreover, the 3HV content reached 20.1?mol%, which is the highest 3HV fraction thus far reported, as for PHBV produced by the wild-type strains grown on unrelated carbon courses. Fermentation experiments suggested that nitrogen-limited MG medium was better than nutrient-rich NOMG and AS168 medium for PHBV production. Additionally, glucose was the most suitable carbon source among the tested carbon sources. Interestingly, PHBV accumulation was almost paralleled by cell growth and glucose consumption. By applying the fed-batch process in fermentor, the PHBV production and cell dry weight were increased by approximately eight and four times, respectively, as compared with those of the batch process in shaking flasks. The classical PHA synthase genes were successfully cloned via consensus-degenerate hybrid oligonucleotide primers (CODEHOPs) and high-efficiency thermal asymmetric interlaced (hiTAIL) PCR methods. This finding suggested that H. amylolyticum shows promising potential in the low-cost biotechnological production of PHBV after further process optimization. ? 2015, Springer-Verlag Berlin Heidelberg.