文献类型：期刊文献

中文题名：液体发酵羊肚菌胞外多糖测定方法的研究

英文题名：Study on extracellular polysaccharide of toadstool produced by liquid fermentation

作者：李蔚[1];赵有玺[2];王忠民[1];龚平[2]

第一作者：李蔚

机构：[1]新疆农业大学食品科学学院,乌鲁木齐830052;[2]北京联合大学生物化学工程学院生物医药系,北京100023

第一机构：新疆农业大学食品科学学院,乌鲁木齐830052

年份：2008

卷号：33

期号：7

起止页码：206-209

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心:【北大核心2004】；

语种：中文

中文关键词：羊肚菌;多糖;分光光度法

外文关键词：toadstool; polysaccharide; spectrophotometer

摘要：测定液态发酵羊肚菌胞外多糖含量时,由于受到培养基中夹带的糖类物质(如淀粉、蔗糖)等物质的干扰,其有效羊肚菌胞外多糖的含量测定可能会受到较大影响。分别以苯酚-硫酸法和改进的ROBERTS铜方法进行羊肚菌胞外多糖测定。实验表明,用ROBERTS铜法所测多糖含量仅占苯酚硫酸法所测多糖含量的17.3%,证明用ROBERTS铜法测定羊肚菌胞外多糖更准确一些。
During the measurement of polysaccharide of toadstool derived from liquid fermentation, the content of the extracellular polysaccharide of toadstool may be interfered by the carbohydrate （such as starch, sucrose） brought from culture medium. In this article, the extracellular polysaccharide is mensurated by two methods： the method of phenol-vitriol and the improved ROBERTS method. It shows that the resulting polysaccharide content by the later method is about 17.3% of that by the former method. It proves that ROBERTS Determination on extracellular Polysaccharide of toadstool is more accurate.