文献类型：期刊文献

中文题名：合生元制剂对小鼠抗氧化能力和肠道菌群的影响

英文题名：Effects of Synbiotics on Antioxidant Capacity and Intestinal Flora in Mice

作者：王涵[1];刘倩[1];郭子晨[1];王韵婷[1];王伶毓[2];赵江燕[2];张连中[3];孙雅煊[1]

第一作者：王涵

机构：[1]北京联合大学生物化学工程学院,北京100023;[2]北京联合大学应用文理学院保健食品检验中心,北京100191;[3]北京鸿测科技发展有限公司,北京100176

第一机构：北京联合大学生物化学工程学院

年份：2022

卷号：43

期号：7

起止页码：1-8

中文期刊名：食品研究与开发

外文期刊名：Food Research and Development

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：国家自然科学基金(11975048);北京联合大学开放课题(SWHX202103);北京联合大学科研项目(XP202007)。

语种：中文

中文关键词：益生菌;肠道菌群;细胞因子;抗氧化;16S r DNA高通量测序

外文关键词：probiotic;intestinal flora;cytokines;antioxidant capacity;16S rDNA high throughput sequencing

摘要：为研究合生元制剂对小鼠抗氧化能力、细胞因子和肠道菌群的影响,随机将48只BALB/c小鼠分为对照组、低剂量组(L)、中剂量组(M)和高剂量组(H),并灌胃生理盐水和不同剂量合生元制剂,14 d后收集小鼠粪便进行菌落计数和16S rDNA高通量测序,测定小鼠肝脏、小肠组织中抗氧化指标,检测血清中细胞因子白介素-6(interleukin-6,IL-6)、白介素-1β(interleukin-1β,IL-1β)水平。结果表明:小肠和肝脏组织中,各个剂量组总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性、谷胱甘肽(glutathione,GSH)含量上升,丙二醛(malondialdehyde,MDA)含量下降。H组IL-1β水平极显著降低(p<0.05)。灌胃14 d后,各剂量组与灌胃前相比,乳杆菌和双歧杆菌数量增加,16S rDNA测序表明给予合生元制剂可以增加Rikenella、Alistipes、Bifidobacterium和Lactobacillus等丰富度。灌胃上述合生元制剂可以提高小鼠抗氧化能力,降低细胞因子水平,促进乳杆菌和双歧杆菌繁殖,对肠道菌群具有调节作用。
This study aimed to test the effects of synbiotics on the antioxidant capacity and intestinal flora of mice.Forty-eight male BALB/c mice were randomly assigned into the control(normal saline)group and lowdose(L),medium-dose(M),and high-dose(H)synbiotic groups and administrated with corresponding agents by gavage.After 14 days,the feces were collected for the characterization of intestinal flora changes by colony counting and 16S rDNA high-throughput sequencing.The antioxidant indexes in the liver and small intestine tissues and the levels of interleukin-6(IL-6)and interleukin-1β(IL-1β)in the serum were measured.In the small intestine and liver tissues,the activities of total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px),and glutathione(glutathione,GSH)rose,and the malondialdehyde(MDA)content reduced after the administration of synbiotics.The level of IL-6 in group H declined(p<0.01).The number of lactobacilli and bifidobacteria in each synbiotics group increased after 14 days of administration.The 16S rDNA high-throughput sequencing results demonstrated that the administration of synbiotics increased the abundance of Rikenella,Alistipes,Bifidobacterium and Lactobacillus.The administration of the above-mentioned synbiotics could improve the antioxidant capacity,reduce the levels of inflammatory cytokines,increase the number of lactobacillus and bifidobacteriain the intestine,and regulate the intestinal flora.