文献类型：期刊文献

中文题名：补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及ECM表达的影响

英文题名：Effect of Tonic Medicine on Kidney Inflammation Factors and ECM Expression in Rats of Exercise-related Renal Ischemia-reperfusion Injuries

作者：郑孟君[1];曹建民[2];郭娴[2];周海涛[3]

第一作者：郑孟君

机构：[1]江南大学体育学院;[2]北京体育大学运动人体科学学院;[3]北京联合大学生物化学工程学院

第一机构：江南大学体育学院,江苏无锡214000

年份：2015

卷号：0

期号：3

起止页码：68-73

中文期刊名：沈阳体育学院学报

外文期刊名：Journal of Shenyang Sport University

收录：国家哲学社会科学学术期刊数据库;北大核心:【北大核心2014】；CSSCI:【CSSCI2014_2016】；

基金：国家体育总局课题(2013A101)

语种：中文

中文关键词：补益中药;运动性肾缺血再灌注损伤;炎症因子;细胞外基质

外文关键词：tonic medicine; exercise-related renal ischemia-reperfusion injury ; inflammation factors ; extracellular matrix

摘要：观察补益中药对运动性肾缺血再灌注损伤大鼠肾脏炎症因子及ECM表达的影响。方法:75只7周龄雄性Wistar大鼠随机分为4组:安静对照组(C组,12只)、一般训练组(M组,12只)、过度训练组(OM组,24只)和补益中药+过度训练组(TM组,24只),M、OM、TM组进行8周56天的游泳训练。采用专业灌胃器每天灌胃1次,TM组灌胃采用黄芪、红景天、丹参、苦参等组成的复合中药制剂,剂量为1 g·kg-1,体积为5 m L·kg-1,其他各组灌胃等量生理盐水。末次训练后24 h,采用PAS染色观察肾小球ECM沉积情况,免疫组织化学法检测肾组织TNF-α、IL-1β、IL-6、IL-18蛋白表达,RT-PCR法检测肾组织TNF-αm RNA、IL-1βm RNA、IL-6 m RNA、IL-18 m RNA、TGF-β1 m RNA表达。结果:1)8周实验后,肾小球ECM沉积,C、M组间无显著差异(P>0.05);OM组较C、M组显著增加(P<0.01);TM组显著低于OM组(P<0.05)。2)血尿素氮和血清肌酐水平,OM组和TM组显著高于C组(P<0.01),TM组显著低于OM组(P<0.05);肾组织TNF-α、IL-1β、IL-6和IL-18蛋白表达,OM组和TM组显著高于C组(P<0.01),TM组显著低于OM组(P<0.05);肾组织TNF-αm RNA、IL-1βm RNA、IL-6 m RNA和IL-18 m RNA表达,OM、TM组显著高于C组(P<0.01),TM组显著低于OM组(P<0.05);肾组织TGF-β1m RNA表达,TM组(P<0.05)和OM组(P<0.01)显著高于C组,TM组显著低于OM组(P<0.05)。结论:8周过度训练致大鼠发生运动性肾缺血再灌注,同时ECM沉积加强。补充补益中药可通过抑制肾脏组织TNF-α、IL-1β、IL-6、IL-18的表达进而减轻了过度训练诱导肾脏缺血再灌注发生时肾脏组织TGF-β1的表达,抑制ECM的合成和(或)促进ECM的降解及组织病理学改变,延缓或避免过度训练导致的运动性缺血再灌注对肾脏的损害。
Objective ： To study the protective effects of tonic medicine on kidney inflammation factors and extracellular ma- trix （ECM） expression in rats of exercise-related renal ischemia-reperfusion injuries. Methods：75 male Wistar rats at the age of 7 weeks were divided into 4 groups randomly：control group （C group, n = 12 ）, general training group （M group, n = 12）, overtralning group（OM group,n = 24）, tonic medicine ＋ overtraining group（ TM group, n = 24）, the Rats in M, OM,TM groups were assigned to 8 weeks of swimming training,7 days a week. Professional gavage were given once a day. The Rats of TM group were given with compound Chinese herbal medicine（ The primary elements were Chinese drugs for invigoration, such as Astragalus, Rhodiola rosea, Salviae miltiorrhizae, Sophora flavescens ）, gavages （ig） dose was 1 g·kg^-1; ig volume was 5 mL ~ kg - l, and other groups were given the normal saline. 24 hours after the last training, we ob- served the glomerular ECM deposition by the PAS stain;detected the renal tissue of TNF-α, IL-1β, IL-6, IL-18 protein by immunohistochemistry and detected the expression of TNF-α mRNA, IL-1β mRNA, IL-6 mRNA, IL-18 mRNA, TGF-β1 mRNA gene in renal tissue by RT-PCR method. Results ： 1 ） 8 weeks after the experiment, the glomerular ECM deposition of C group and M group appeared normal and showed no differences （ P 〉 0.05 ） ; OM group was significantly higher than that in C group and M group（ P 〈 0.01 ）, TM group was significantly lower than that of group OM （ P 〈 0.05 ）. 2） OM group and TM group blood urea nitrogen and serum creatinine were significantly higher than that of C group（ P 〈 0.01 ）, TM group was significantly lower than that of group OM （ P 〈 0.05 ） ; the expression of renal TNF-α, IL-1 13, IL=6 and IL-18 protein in OM group and TM group was significantly higher than that in C group （P 〈 0.01 ）, TM group was significantly lower than that of group OM（P 〈0.05） ;the expression of renal TNF-α mRNA,IL-1β mRNA,IL-6 and IL-18 mRNA in TM group and OM group was significantly higher than that of C group（P 〈0.01 ） ,TM group was lower than of OM group（ P 〈 0.05 ） ;the expression of renal TGF-β1 mRNA in TM group and OM group was significantly higher than that of C group （ TM, P 〈 0.05 ; OM, P 〈 0.01 ）, TM group was significantly lower than of OM group （ P 〈 0.05 ）. Conclusion： 8 weeks ovettraining caused the movement of rat renal ischemia reperfusion and increased the deposition of ECM. Tonic medicine can restrain the expression of renal tissue TGF-1β during exercise-related renal ischemia-repeffusion injury in- duced by overtraining, which may be achieved by inhibiting the expression of IL-1, TNF-α, IL-6, IL-18 in renal tissue. Finally it decreased the synthetic and increased the decomposition of ECM, reduced the damage and the histopathological changes, as a result, Tonic medicine can protect the rats from exercise-related renal ischemia-reperfusion injury.