文献类型：期刊文献

英文题名：Mutagenic and teratogenic toxicity evaluation of Forsythia suspensa leaves aqueous extract

作者：Liu, Yinlu[1];Zhao, Jian[1];Guo, Yu[1];Wang, Meng[2];Li, Xiaoyan[2];Zhang, Bo[1]

第一作者：刘亚林

通讯作者：Zhang, B[1]

机构：[1]Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China;[2]Shijiazhuang Yiling Pharmaceut Co Ltd, Shijiazhuang, Hebei, Peoples R China

第一机构：北京联合大学生物化学工程学院|北京联合大学应用文理学院

通讯机构：[1]corresponding author), Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2022

卷号：45

期号：4

起止页码：1825-1832

外文期刊名：DRUG AND CHEMICAL TOXICOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000618310500001)】；

基金：This project is funded by Shijiazhuang Yiling Pharmaceutical Co. and Beijing Union University Research and Innovation Funding Project [YZ2020K001].

语种：英文

外文关键词：Forsythia suspensa leaves; aqueous extract; genotoxicity; teratogenicity; safety

摘要：Forsythia suspensa leaves (FSL), rich in phillyrin, forsythiaside A, phillygenin, rutin, and other compounds, is a known traditional Chinese medicine (TCM). It has been effective in heat retreat and detoxification. In this study, we performed the mutagenic and teratogenic toxicity evaluation of FSL aqueous extract (FSLAE) using the bacterial reverse mutation assay (Ames test), mouse bone marrow micronucleus assay, spermatocyte chromosomal aberration assay in mice. Kunming mice and SD rats were used were for the mutagenic and the teratogenic studies, respectively. We found that FSLAE was not mutagenic and did not induce unfavorable chromosomal events. Additionally, the Ames test revealed FSLAE was not genotoxic and showed no mutagenic activity in histidine dependent strains of Salmonella typhimurium at concentrations up to 5000 mu g/plate. Likewise, in vivo test revealed no induced micronucleus of mouse bone marrow or chromosome aberration in spermatocytes up to the dose of 10.00 g/kg BW. For the teratogenic evaluations, pregnant rats were treated with 1.04, 2.08, and 4.17 g/kg FSL, and fetuses were examined on the 6-15 day of pregnancy. We observed no maternal toxicity and embryotoxicity related to the treatment. Based on these in vitro and in vivo studies, we concluded the genotoxic and teratogenic safety of FSL.