文献类型：期刊文献

英文题名：Recent advances in the biosynthesis of isoprenoids in engineered Saccharomyces cerevisiae

作者：Wang, Zhaobao[1];Zhang, Rubing[2];Yang, Qun[1];Zhang, Jintian[3];Zhao, Youxi[3];Zheng, Yanning[4];Yang, Jianming[1]

第一作者：Wang, Zhaobao

通讯作者：Yang, JM[1]

机构：[1]Qingdao Agr Univ, Coll Life Sci, Shandong Key Lab Appl Mycol, Energy Rich Cpds Prod Photosynthet Carbon Fixat R, Qingdao, Peoples R China;[2]Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China;[3]Beijing Union Univ, Coll Biochem Engn, Beijing, Peoples R China;[4]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China

第一机构：Qingdao Agr Univ, Coll Life Sci, Shandong Key Lab Appl Mycol, Energy Rich Cpds Prod Photosynthet Carbon Fixat R, Qingdao, Peoples R China

通讯机构：[1]corresponding author), Qingdao Agr Univ, Coll Life Sci, Shandong Key Lab Appl Mycol, Energy Rich Cpds Prod Photosynthet Carbon Fixat R, Qingdao, Peoples R China.

年份：2021

卷号：114

起止页码：1-35

外文期刊名：ADVANCES IN APPLIED MICROBIOLOGY, VOL 114

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000748724500001)】；

基金：This work was supported by grants from the "First class grassland science discipline" program in Shandong Province, CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (BMF-2020-01), the Talents of High Level Scientific Research Foundation (grants 6651117005 and 6651119011) of Qingdao Agricultural University, Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CASKLB201805).

语种：英文

摘要：Isoprenoids, as the largest group of chemicals in the domains of life, constitute more than 50,000 members. These compounds consist of different numbers of isoprene units (C5H8), by which they are typically classified into hemiterpenoids (C5), monoterpenoids (C10), sesquiterpenoids (C15), diterpenoids (C20), triterpenoids (C30), and tetraterpenoids (C40). In recent years, isoprenoids have been employed as food additives, in the pharmaceutical industry, as advanced biofuels, and so on. To realize the sufficient and efficient production of valuable isoprenoids on an industrial scale, fermentation using engineered microorganisms is a promising strategy compared to traditional plant extraction and chemical synthesis. Due to the advantages of mature genetic manipulation, robustness and applicability to industrial bioprocesses, Saccharomyces cerevisiae has become an attractive microbial host for biochemical production, including that of various isoprenoids. In this review, we summarized the advances in the biosynthesis of isoprenoids in engineered S. cerevisiae over several decades, including synthetic pathway engineering, microbial host engineering, and central carbon pathway engineering. Furthermore, the challenges and corresponding strategies towards improving isoprenoid production in engineered S. cerevisiae were also summarized. Finally, suggestions and directions for isoprenoid production in engineered S. cerevisiae in the future are discussed.