文献类型：期刊文献

中文题名：低聚原花青素对过度训练大鼠骨骼肌损伤的保护作用机制

英文题名：Protective Effect of Oligomerized Proanthocyanidins on Skeletal Muscle Injury in Overtraining Rats

作者：周海涛[1,2];曹建民[3];胡戈[4];牛衍龙[5];龚平[1];邵芙蓉[1];张子坤[1];黄景宣[1];董爱霞[1]

第一作者：周海涛

机构：[1]北京联合大学生物化学工程学院,中国北京100023;[2]北京联合大学生物活性物质与功能食品北京市重点实验室,中国北京100191;[3]北京体育大学运动人体科学学院,中国北京100084;[4]常州大学,中国江苏常州213164;[5]赣南医学院,中国江西赣州341000

第一机构：北京联合大学生物化学工程学院

年份：2021

卷号：25

期号：1

起止页码：24-30

中文期刊名：生命科学研究

外文期刊名：Life Science Research

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：北京市朝阳区协同创新项目(CYXC1817);北京市高等学校高水平人才交叉培养“实培计划”项目;北京联合大学2019年“启明星”大学生科技创新创业项目(201911417SJ155);北京联合大学科研项目(ZK70202005);赣南医学院校级重点课题(ZD201812);赣南医学院博士启动基金课题(QD201817)。

语种：中文

中文关键词：低聚原花青素(OPC);过度训练;骨骼肌损伤;氧化应激;p38丝裂原激活的蛋白激酶(p38 MAPK);大鼠

外文关键词：oligomerized proanthocyanidins(OPC);overtraining;skeletal muscle injury;oxidative stress;p38 mitogen-activated protein kinase(p38 MAPK);rat

摘要：为研究低聚原花青素对过度训练大鼠骨骼肌损伤的保护作用机制,将大鼠随机分为安静对照组(C)、过度训练组(OM)、低聚原花青素干预组(OOM)。C组无运动干预,其他组采用42 d递增负荷跑台训练。训练期间, OOM组每天灌胃低聚原花青素1次(150 mg/kg, 5 m L/kg),其他组给予等体积蒸馏水。末次训练后即刻取材,经HE染色后观察骨骼肌组织形态,采用免疫组化法、酶联免疫法、放射免疫法等方法检测相关生化指标:血清睾酮(testosterone, T)、皮质酮(corticosterone, Cor)、肌酸激酶(creatine kinase, CK)和乳酸脱氢酶(lactate dehydrogenase, LDH);骨骼肌p38丝裂原激活的蛋白激酶(p38 mitogen-activated protein kinase, p38 MAPK)及其磷酸化蛋白质、超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、3-硝基酪氨酸(3-nitrotyrosine, 3-NT)和8-羟基脱氧鸟苷(8-hydroxy-2-deoxyguanosine, 8-OHdG)。结果显示:低聚原花青素干预可以改善过度训练所致骨骼肌组织形态学改变,显著提高血清T/Cor比值及骨骼肌SOD活性(P<0.01),降低骨骼肌p38 MAPK/p-p38 MAPK水平、血清CK活性(P<0.05)及骨骼肌MDA、3-NT、8-OHdG含量(P<0.05或P<0.01)。实验结果表明低聚原花青素通过改善氧化应激,调控p38 MAPK信号通路相关蛋白质表达,保护过度训练大鼠骨骼肌结构/功能。
In order to study the protective mechanism of oligomerized proanthocyanidins(OPC)on skeletal muscle injury in overtraining rats,the rats were randomly assigned to control group(C),overtraining group(OM),OPC treatment combined with overtraining group(OOM).There was no exercise intervention in C group,and the other groups underwent 42-day incremental load treadmill exercise to establish skeletal muscle injury model caused by overtraining.During the training period,OOM group was administered intragastrically with OPC once a day(150 mg/kg,5 mL/kg),and the other groups were given an equal volume of distilled water.The rats were sacrificed immediately after the last training,and the morphology of skeletal muscle was observed by HE staining.Immunohistochemistry,enzyme-linked immunosorbent assay,radioimmunoassay and other methods were used to detect relevant biochemical indicators:testosterone(T),corticosterone(Cor),creatine kinase(CK)and lactate dehydrogenase(LDH)in serum,p38 mitogen-activated protein kinase(p38 MAPK)and its phosphorylated protein,superoxide dismutase(SOD),malondialdehyde(MDA),3-nitrotyrosine(3-NT)and 8-hydroxy-2-deoxyguanosine(8-OHdG)in skeletal muscle.The results showed that OPC could improve morphological changes of skeletal muscle caused by overtraining,increase serum T/Cor ratio and SOD activity in skeletal muscle significantly(P<0.01),reduce p38 MAPK/p-p38 MAPK expression in skeletal muscle,serum CK activity(P<0.05),MDA,3-NT and 8-OHdG contents in skeletal muscle significantly(P<0.05 or P<0.01).The above results indicate that OPC can improve oxidative stress and regulate p38 MAPK signaling pathwayrelated protein expression,thereby protecting skeletal muscle structure and function in over-training rats.