文献类型：期刊文献

中文题名：白菜叶际细菌多样性与毒死蜱降解菌筛选及分离鉴定

英文题名：Diversity of Bacteria,Isolation and Identification of Chlorpyrifos-degradating Strains on the Phyllosphere of Brassica chinensis

作者：陈琳[1,2];祁静[1];李祖明[1];白志辉[3];高丽萍[1]

第一作者：陈琳

机构：[1]北京联合大学生物化学工程学院;[2]中国科学院大学资源与环境学院;[3]中国科学院生态环境研究中心

第一机构：北京联合大学生物化学工程学院

年份：2018

卷号：39

期号：22

起止页码：107-112

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金面上项目(31570494);国家留学基金委项目(201708110129);北京市自然科学基金项目(6173033);校级科技成果转化培育项目(22213591113/001)

语种：中文

中文关键词：白菜;叶际细菌;多样性;毒死蜱降解菌;筛选;鉴定;QuEChERS-气相色谱-质谱联用

外文关键词：Brassica chinensis;phyllospheric bacteria;diversity;chlorpyrifos - degradating strains;isolation;identification;QuEChERS-GC/MS

摘要：研究白菜叶际细菌多样性,并从白菜叶际筛选鉴定毒死蜱降解菌。分别采用LB、PDA、改良高氏一号和毒死蜱(100 mg/L)无机盐培养基对白菜叶际上细菌进行分离筛选,得到其细菌浓度分别为1.24×10~6、1.03×10~4、2.07×10~4和2.64×10~5CFU/g,表明白菜叶际细菌在数量上存在多样性,共筛选到51株表型差异较明显的细菌。经16S rDNA分子鉴定表明各培养基上优势菌分别是肠杆菌属(Enterobacter)、肠杆菌属(Enterobacter)、节杆菌属(Anthrobacter)和假单孢菌属(Pseudomonas)分别约占各自培养基所分离鉴定菌株的28%、75%、100%和55%。QuEChERS-气相色谱-质谱联用法检测到毒死蜱降解率较高的细菌有11株,其中降解率最高的菌株是沙雷氏菌(Serratia ureilytica) M1,达到52.44%。研究发现,不同培养基从白菜叶际分离鉴定的细菌多样性差异明显,本研究从白菜叶际筛选鉴定出毒死蜱降解菌率较高的细菌菌株,为毒死蜱降解菌的降解特性、应用和机理研究提供了基础。
Diversity of bacteria, isolation and identification of chlorpyrifos- degradating strains on the phyllosphere of Brassica chinensis were studied in the paper. The bacteria on the phyllosphere of Brassica chinensis were seperated by LB culture medium, PDA culture medium, high one medium and inorganic salt medium contained 100 mg/L chlorpyrifos, and their number of bacteria were 1.24 × 10^6 ,1.03 ×10^4 ,2.07 × 10^4 and 2.64 × 10^5 CFU/g respevtively,which showed the diversity of bacteria number.There were 51 strains of bacteria with obviously phenotype were selected.Their dominant bacterias on each medium were Enterobacter（ 28.0% ） , Enterobacter （ 75.0% ） ,Arthrobacter （ 100.0% ） and Pseudomonas （ 55.0% ） by identification of 16S rDNA with PCR respectively.Their degradation rate of chlorpyrifos were determined by QuEChERS-GC/MS, and 11 strains were isolated with good degradation activities to chlorpyrifos.The maximum rate of chlorpyrifos degradation was 52.44% , the strain was Serratia ureilytica M1.This study found that diversity of bacteria which was isolated and identified from phyllosphere of Brassica chinensis by various culture medium was obviously different, and chlorpyrifos-highly-degradating strains were also isolated and identified from phyllosphere of Brassica chinensis, which provided a basis for the study of degradation characteristics,application and mechanism of chlorpyrifos-degradating strains.