文献类型：期刊文献

中文题名：响应面法优化肉苁蓉水提物的制备工艺及其活性研究

英文题名：Optimization of Aqueous Extraction Preparation Technology of Cistanche deserticola by Response Surface Methodology and Its Activity Research

作者：冯朵[1];常想[1];蒋勇军[2];郭豫[1];赵建[1];赵江燕[1];孙巧弟[1];松伟[1];崔倩倩[1];闫文杰[1]

第一作者：冯朵

机构：[1]北京联合大学生物化学工程学院,生物活性物质与功能食品北京市重点实验室,北京100023;[2]内蒙古三口生物科技有限公司,内蒙古鄂尔多斯017000

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院

年份：2023

卷号：44

期号：5

起止页码：139-148

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;EI(收录号：20241015701139);Scopus(收录号：2-s2.0-85148534722);北大核心:【北大核心2020】；

基金：北京市属高校分类发展项目;北京联合大学科研项目(JB202101)。

语种：中文

中文关键词：荒漠肉苁蓉;水提法;响应面分析;抗氧化活性

外文关键词：Cistanche deserticola Y.C.Ma;water extraction;response surface analysis;antioxidant activity

摘要：为了研究肉苁蓉水提物制备工艺及其营养价值和活性功能研究,本实验采用水提取法,考察不同提取条件对肉苁蓉浸膏得率的影响,并进一步采用响应面法优化工艺,再测定提取物营养成分及功效成分含量,讨论其抗氧化功能及对秀丽隐杆线虫寿命和生殖力的影响。结果显示,当料液比为1:12 g/mL,煎煮时间为140 min,煎煮次数为4次,肉苁蓉水提物(Aqueous extract of Cistanche,AEC)浸膏得率为65.83%±0.87%。此外,检测AEC的营养成分和活性成分,发现其含有大量的营养成分,例如蛋白质(5.22±0.44 g/100 g)、脂肪(0.150±0.06 g/100 g)、氨基酸(0.640±0.05 g/100 g)等,以及松果菊苷(557±4.24 mg/100 g)、毛蕊花糖苷(169±7.07 mg/100 g)、总糖(22.1±0.64 g/100 g)、总黄酮(4.13±0.08 g/100 g)等多种功效成分。AEC还具有良好的DPPH·清除能力、抑制·OH能力、总抗氧化能力和还原能力,且均呈浓度依赖性。最后,AEC还可以延长秀丽隐杆线虫的寿命,其中8 mg/mL AEC效果最好,平均寿命可达10.95±2.40 d。由此可见,水提取后的AEC具有良好的抗氧化能力,富含多种营养成分和功效成分,这提示肉苁蓉具备开发食品抗氧化剂或功能食品的潜力,为日后肉苁蓉的临床应用研究提供一定的理论依据。
To study the nutritional value and antioxidant activities of Cistanche deserticola Y.C.Ma extract.The water extraction method was used in this experiment to research the effects of different extraction conditions on the dry extract rate of Cistanche deserticola Y.C.Ma.Then the response surface analysis method was further used to optimize the process.Moreover,the nutritional components and functional components of the extract were determined,and the antioxidant function and effects on the life span and fecundity of C.elegans were discussed.The results showed that when the material liquid ratio was 1:12 g/mL,the decocting time was 140 min,and the decocting times were 4 times,the dry extract yield of the aqueous extract of Cistanche(AEC)was 65.83%±0.87%.In addition,the nutritional composition and functional components of AEC was detected and found to contain a large amount of nutritional components such as protein(5.22±0.44 g/100 g),fat(0.150±0.06 g/100 g),amino acids(0.640±0.05 g/100 g),etc,and echinacoside(557±4.24 mg/100 g),acteoside(169±7.07 mg/100 g),total sugar(22.1±0.64 g/100 g),total flavonoids(4.13±0.08 g/100 g)and others.Furthermore,the AEC also showed good DPPH scavenging activity,hydroxyl radical scavenging activity,total antioxidant activity,and reducing activity,all of which were concentration dependent.Finally,AEC could prolong the life span of Caenorhabditis elegans.Among them,8 mg/mL AEC had the best effect,and its average life span reached 10.95±2.40 d.Therefore,AEC has good antioxidant capacity,and rich in various nutrients and functional components,which indicates that Cistanche deserticola Y.C.Ma has the potential to develop functional food antioxidants and provides a theoretical basis for the clinical application of Cistanche deserticola Y.C.Ma in the future.