文献类型：期刊文献

英文题名：Multi-module engineering to guide the development of an efficient L-threonine-producing cell factory

作者：Zhao, Zhenqiang[1,2];You, Jiajia[1,2];Shi, Xuanping[1,2];Cai, Mengmeng[1,2];Zhu, Rongshuai[1,2];Yang, Fengyu[1,2];Xu, Meijuan[1,2];Shao, Minglong[1,2];Zhang, Rongzhen[1];Zhao, Youxi[1,3];Rao, Zhiming[1,2]

第一作者：Zhao, Zhenqiang

通讯作者：Zhao, YX[1];Rao, ZM[1]

机构：[1]Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China;[2]JITRI, Inst Future Food Technol, Yixing 214200, Peoples R China;[3]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China

第一机构：Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China

通讯机构：[1]corresponding author), Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China.

年份：2025

卷号：416

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20244617365840);Scopus(收录号：2-s2.0-85208763510);WOS:【SCI-EXPANDED(收录号:WOS:001358513900001)】；

基金：The National Key Research and Development Program of China (2021YFC2100900) . The National Natural Science Foundation of China (32071470) . The National First-class Discipline Program of Light Industry Technology and Engineering (QGJC20230201) . The Major Scientific and Technological Project for "unveiling and commanding" of Hohhot (2023-unveiling and commanding-He-1) . The Program of Introducing Talents of Discipline to Universities (111-2-06) and Post-graduate Research & Practice Innovation Program of Jiangsu Provence (KYCX22-2369) .

语种：英文

外文关键词：Modular metabolic engineering; L-threonine; CO 2 capture and fixation; Quorum-sensing

摘要：The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multimodule metabolic engineering was implemented to reprogram Escherichia coli, enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO2 was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of Lthreonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for de novo producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.