文献类型：期刊文献

中文题名：基于α-葡萄糖苷酶抑制率的桦褐孔菌多糖提取工艺优化

英文题名：Optimization the extraction process of polysaccharide from Inonotus obliquus based on the inhibition rate of α-glucosidase

作者：王昭润[1,2];刘萍[2];荣瑞芬[3];吴晓彤[1]

第一作者：王昭润

机构：[1]内蒙古大学生命科学学院;[2]中国农业大学食品科学与营养工程学院;[3]北京联合大学生物化学工程学院

第一机构：内蒙古大学生命科学学院,内蒙古呼和浩特010020

年份：2018

卷号：37

期号：4

起止页码：150-154

中文期刊名：中国酿造

外文期刊名：China Brewing

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：国家自然科学基金(21576282)

语种：中文

中文关键词：桦褐孔菌;多糖;α-葡萄糖苷酶抑制率;提取工艺;优化

外文关键词：Inonotus obliquus;polysaccharides;α-glucosidase inhibition rate;extraction process;optimization

摘要：为获得高活性桦褐孔菌多糖提取工艺,以多糖提取率和α-葡萄糖苷酶抑制率为考察指标,在单因素试验的基础上,利用响应面法优化提取工艺。结果表明,高活性多糖最佳提取工艺为料液比1∶30(g∶mL),提取温度95℃,提取时间3.5h,醇沉倍数2倍,在此条件下,多糖提取率为4.61%,α-葡萄糖苷酶抑制率为92.34%。
In order to obtain the polysaccharide extraction process of Inonotus obliquus with high activity,with polysaccharide extraction rate and a-glucosidase inhibition rate as investigate indexes,on the basis of single factor experiments,the extraction process was optimized by response surface methodology.The results showed that the optimum extraction conditions of the high activity polysaccharide were solid-liquid ratio 1-30(g:ml),extraction temperature 95℃.,time 3.5 h,alcohol precipitation multiple 2.Under these conditions,the polysaccharide extraction rate was 4.61%and the a-glucosidase inhibition rate was 92.34%.