文献类型：期刊文献

中文题名：神经干细胞的三维培养及其在神经疾病中应用的研究进展

英文题名：Progress in research on three dimensional culture of neural stem cells and its application in neurological diseases

作者：赵范范[1];章丽娜[1];商迎辉(综述)[1];劳凤学(审校)[1]

第一作者：赵范范

机构：[1]北京市生物活性物质和功能食品重点实验室,北京联合大学生物化学与工程学院,北京100191

第一机构：北京联合大学生物化学工程学院

年份：2020

卷号：33

期号：2

起止页码：227-231

中文期刊名：中国生物制品学杂志

外文期刊名：Chinese Journal of Biologicals

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2019_2020】；

语种：中文

中文关键词：神经干细胞;三维培养;神经系统疾病

外文关键词：Neural stem cells(NSCs);Three-dimensional(3D)culture;Neurological disease

摘要：作为从二维(two-dimensional,2D)培养衍生出的新技术,神经干细胞(neural stem cells,NSCs)的三维(threedimensional,3D)培养不仅能够模拟脑内微环境,更好地维持NSCs增殖及分化能力,还能进一步形成脑类器官。3D培养NSCs及脑类器官均可作为有效的体外细胞模型,不仅能模拟神经疾病的发病过程,还可应用于高通量药物筛选;另外,利用NSCs移植及定向分化等特征修复和替代死亡的神经细胞,可延缓或抑制阿尔茨海默症(alzheimer disease,AD)等神经系统疾病。NSCs的3D培养将有助于研究者更好地理解大脑发育过程,以及神经系统疾病特别是神经退行性疾病的发病机制,同时有助于寻找药物靶点和药物开发。由此可见NSCs的3D培养展现出巨大的优势,具有良好的应用前景。本文对NSCs的特性和3D培养方式,及其在神经疾病中应用的最新研究进展作一综述。
As a new technology derived from two-dimensional(2D)culture,the three-dimensional(3D)culture of neural stem cells(NSCs)can not only simulate the microenvironment of brain,but also maintain the ability of proliferation and differentiation of stem cells,and further form brain organs. Both the 3D cultures of NSCs and brain organs may be used as cell models in vitro to simulate the pathogenesis of neurological diseases and to screen the high-thought drugs. In addition,because of their biological characteristics of differentiation and migration,NSCs may be used to repair and replace the dead neural cells,which may provide a new idea for the prevention and treatment of neurological diseases such as Alzheimer disease. The 3D culture of NSCs will be helpful to further understanding the brain development process and the pathogenesis of neurological diseases especially neurodegenerative diseases,and to the finding of drug targets and the development of drugs. It can be seen that the 3D culture of NSCs shows great advantages and has a good application prospect. This review summarizes the characteristics of NSCs and 3D culture methods as well as the latest progress in research on application of 3D culture in neurological diseases.