文献类型：期刊文献

英文题名：Production of 1,3-propanediol from glycerol using a new isolate Klebsiella sp. AA405 carrying low levels of virulence factors

作者：Li, Ying[1,2]; Ge, Xi-Zhen[2]; Tian, Ping-Fang[1]

第一作者：Li, Ying;李映

通讯作者：Tian, Ping-Fang

机构：[1] Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China; [2] College of Biochemical Engineering, Beijing Union University, Beijing, China

第一机构：Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

通讯机构：[1]Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

年份：2017

卷号：31

期号：4

起止页码：725-732

外文期刊名：Biotechnology and Biotechnological Equipment

收录：EI(收录号：20172303734831);Scopus(收录号：2-s2.0-85020198690)

语种：英文

外文关键词：Carbon - Industrial chemicals - Escherichia coli

摘要：Klebsiella pneumoniae is a promising industrial species for the production of chemicals, yet its opportunistic pathogenicity restrains real-world applications. In this work, we identified a novel isolate designated Klebsiella sp. AA405 which carried low levels of virulence factors. No capsule, fimbriae and flagella were observed around colonies. Genome alignment between Klebsiella sp. AA405 and other 186 Klebsiella species revealed 3421 homologous genes. Notably, the endotoxin of Klebsiella sp. AA405 was only half of E. coli BL21. This strain matched well with common cloning and expression vectors and was sensitive to antibiotics. More importantly, this strain metabolized diverse carbon sources. Under micro-aerobic conditions, it produced 51.66 g/L 1,3-propanediol, 3.82 g/L 3-hydroxypropionic acid and 6.86 g/L 2,3-butanediol in a 5 L bioreactor using glycerol as the sole carbon source. Overall these results indicate that Klebsiella sp. AA405 is a promising strain for the production of bulk chemicals. ? 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.