文献类型：期刊文献

英文题名：Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

作者：Li, Ying[1];Chi, Miaomiao[1];Ge, Xizhen[1]

第一作者：李映

通讯作者：Ge, XZ[1]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing Key Lab ofBiomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：12

期号：2

起止页码：218-224

外文期刊名：INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000464947900027)】；

基金：This work was fmancially supported by Joint Funding of Beijing Municipal Natural Science Foundation -Beijing Municipal Education Commission (KZ201911417049), National Key R&D Program of China (2017YED0201105) and Premium Funding Project for Academic Human Resources Development in Beijing Union University (BPHR2017DZ07).

语种：英文

外文关键词：carbendazim; bioremediation; antifungal and antibacterial pesticide; hydrolase; soil; food; pesticide contamination

摘要：Carbendazim (MBC) is an effective antifungal and antibacterial pesticide in agricultural applications. However, the MBC-contaminated soil and food are difficult to be restored. In this work, a novel MBC-hydrolase HY-1 encoded by gene by-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified. The 858 bp hydrolase gene was expressed in E. coli BL21 and the 32 kDa hydrolase HY-1 was purified. The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole (2-AB) without the need for any cofactors. Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E. coli (pET-hy1) accelerated MBC degradation in soil. Moreover, purified HY-1 was available in removing MBC residue on the surface of cucumber. This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food, provide a new way for bioremediation of pesticide contaminations.