文献类型：期刊文献

中文题名：魔芋低聚糖对结肠炎大鼠肠道菌群的影响

英文题名：Effect of Konja Coligosaccharide on Gut Microbiota in Rats with Ulcerative Colitis

作者：刘瑞雪[1];李勇超[1];张波[1]

第一作者：刘瑞雪

通讯作者：Zhang, Bo

机构：[1]北京联合大学功能食品科学技术研究院北京联合大学应用文理学院

第一机构：北京联合大学应用文理学院|北京联合大学生物化学工程学院功能食品科学技术研究院

年份：2017

卷号：17

期号：6

起止页码：53-59

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute of Food Science and Technology

收录：CSTPCD;;EI(收录号：20174304306389);Scopus(收录号：2-s2.0-85031938923);北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金："十二五"国家科技支撑计划课题(2012BAD33B06)

语种：中文

中文关键词：魔芋低聚糖;溃疡性结肠炎;肠道菌群;短链脂肪酸

外文关键词：konja coligosaccharide; Ulcerative colitis; gut microbiota; short-chain fatty acids;

摘要：目的:研究魔芋低聚糖(KOS)对溃疡性结肠炎大鼠肠道菌群结构的影响,并探讨其改善大鼠结肠炎症的可能作用机制。方法:采用2,4,6-三硝基苯磺酸(TNBS)诱导大鼠溃疡性结肠炎模型,40只SD大鼠随机被分为4组:正常组、模型组、KOS低剂量组(灌胃1 g/(kg·d))、KOS高剂量组(灌胃4 g/(kg·d))。每日对大鼠进行临床活动度观察,14 d后10%水合氯醛麻醉处死大鼠,无菌收集结肠内容物,制作粪涂片进行菌群失调分度检验,涂布平板法测大肠杆菌、肠球菌、双歧杆菌、乳酸杆菌的数量,气相色谱(GC)检测乙酸、丙酸、丁酸含量。结果:低、高剂量KOS组均明显缓解溃疡性结肠炎大鼠临床症状,改善菌群失调状况,显著提高双歧杆菌、乳杆菌的数量,降低大肠杆菌、肠球菌的数量,并能显著提高短链脂肪酸(SCFA)的产量。结论:魔芋低聚糖可通过调节肠道菌群的结构与功能对溃疡性结肠炎起干预与治疗作用。
Objective： To explore effect of konja coligosaccharide（KOS） on gut microbiota in Rats with Ulcerative colitis and to explore possible mechanisms involved. Methods： Experimental rat colitis was induced by trinitrobenzenesulfonic acid（TNBS） and randomly distributed into four groups as follows： normal control group, model control group,KOS low dose group and high dose group. Assessment of colitis severity was performed daily. All rats were killed on the14 st day and their colon contents were dissected and collected： spread plate method was used to detect the content of feces Escherichia coli, Enterococcus, Bifidobacterium and Lactobacillus. Gas chromatography to determinate the types and contents of short-chain fatty acids. Results： Compared with the model group, KOS low and high dose group reduced the disease activity index score, improved the structure and function of gut microbiota： the Escherichia coli and Enterococcus decreased significantly, the Bifidobacterium and Lactobacillus have great proliferation and short-chain fatty acids increased significantly. Conclusion： The mechanism of KOS in relieving inflammation in colitis may be achieved by improving the structure and function of gut microbiota.