文献类型：期刊文献

英文题名：Total glycosides of Cistanche deserticola enhance the inhibitory effect of Sorafenib on mice with hepatocellular carcinoma

作者：Feng, Duo[1,2,3];Wang, Jing[3];Zhang, Shaoshi[1,2];Zhou, Shiqi[1,2];Li, Mengjie[1,3];Wang, Xianchun[4];Guo, Yu[1];Zhao, Jian[1];Yan, Wenjie[1,2]

第一作者：Feng, Duo

通讯作者：Yan, WJ[1];Yan, WJ[2]

机构：[1]Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100023, Peoples R China;[3]Minist Agr & Rural Affairs, Inst Food & Nutr Dev, Beijing 100081, Peoples R China;[4]Adm Market Regulat Dongsheng Dist, Ordos 017000, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Union Univ, Coll Biochem Engn, Beijing Key Lab Bioact Subst & Funct Food, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;[114172]北京联合大学应用文理学院;

年份：2026

卷号：15

期号：7

外文期刊名：FOOD SCIENCE AND HUMAN WELLNESS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001843599700001)】；

基金：This work was supported by the National Key Research and Development Program of China (2023YFF1103800), Key and Major Cultivation Project of Beijing Union University (ZK10202501) and Key Technology Research on Identification of Risk Factors and Systematic Evaluation of Edible Safety for Animal-derived Novel Food Resources (2023YFF1103803).

语种：英文

外文关键词：Cistanche deserticola; Anti-hepatocellular carcinoma; Total glycosides; Sorafenib; Wnt/beta-catenin signaling pathway

摘要：Long-term use of Sorafenib (SOR) in patients with hepatocellular carcinoma will lead to drug resistance, leading to diarrhea, weight loss and other adverse reactions. Cistanche deserticola, as a homologous substance of medicine and food, its total glycosides (TG) have anti-hepatocellular carcinoma activity. This paper aimed to explore the anti-hepatocellular carcinoma effect of TG combined with SOR on mice and its mechanism. In this study, according to the previous research of experts, the dosage of 30 mg/kg SOR was selected, aiming at giving consideration to the anti-tumor effect and reducing toxicity. Specifically, HepG2 cells were used to establish a subcutaneous tumorigenic model. Histological changes, oxidative stress, immune regulation and intestinal metabolism and microbial composition of mice were detected. It was found TG did not affect the normal growth of mice without any toxic side effects and could synergize with SOR to inhibit tumor growth. Hematoxylin-eosin results found mice in TG group had more complete hepatocyte structure and less pathological changes in liver. The tumor tissue cells became rare and necrotic. TUNEL staining results revealed that TG could significantly promote apoptosis of HepG2 cells, but at a lower level than SOR. In addition, the content of catalase (CAT), superoxide dismutase (SOD) and glutamic-pyruvic transaminase (ALT) increased, aspartate aminotransferase (AST) and alkaline phosphatase (ALP) decreased, indicating TG could effectively reduce liver lesions. TG could also decrease the content of interleukin-2 (IL-2), interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-alpha), etc., and increase interferon-gamma (IFN-gamma), granulocyte-macrophage colony stimulating factor (GM-CSF). Meanwhile, immunohistochemistry was used to observe the decreased expression of Ki67, beta-catenin and dishevelled (Dsh) proteins and the increased GSK-3 beta in the tumor tissues, it was presumed that TG exerted its anti-hepatocellular carcinoma activity through the Wnt/beta-catenin signaling pathway. Most importantly, TG could synergize with SOR to regulate metabolic levels in the gut, balance intestinal microbial composition, and increase the abundance of beneficial intestinal bacteria. In conclusion, the results showed that TG (64 mg/kg) could assist SOR (30 mg/kg) to inhibit the development of hepatocellular carcinoma, provide some theoretical basis and technical support. C. deserticola, as a homologous substance of medicine and food, its combination with SOR can provide a new idea for the treatment of hepatocellular carcinoma and its future application potential is worth further exploration and development.