文献类型：期刊文献

中文题名：利用果渣产黄腐酸菌剂的筛选

英文题名：Screening of strains for decomposing fruit residues to produce fulvic acid

作者：冀颐之[1];赵有玺[1];彭雪菲[1];李思宇[1];杜军[1,2]

第一作者：冀颐之

机构：[1]北京联合大学生物化学工程学院生物质废弃物资源化利用北京市重点实验室,北京100023;[2]中国生物发酵产业协会,北京100833

第一机构：北京联合大学生物化学工程学院生物医药系

年份：2019

卷号：17

期号：5

起止页码：530-535

中文期刊名：生物加工过程

外文期刊名：Chinese Journal of Bioprocess Engineering

收录：CSTPCD

基金：北京市教委面上项目(KM201311417004);北京联合大学人才强校优选计划(BPHR2017CZ06);生物质废弃物资源化利用北京市重点实验室开放课题

语种：中文

中文关键词：黄腐酸;果渣;木质纤维素;发酵;16SrDNA

外文关键词：fulvic acid;fruit residues;lignocellulose;fermentation;16S rDNA

摘要：以果渣发酵生产黄腐酸可实现农业有机固定废弃物的资源化利用。从腐植酸样品中分离获得4株耐高温菌,以果渣为基质发酵生产黄腐酸,通过测定黄腐酸产量和木质纤维素降解率,从中筛选出2株能够较好降解木质纤维素的黄腐酸生产菌株BFA02和BFA03,其黄腐酸产量分别为43.67、59.35g/kg。经16SrDNA分类鉴定,初步确认BFA02为解淀粉芽孢杆菌,BFA03为地衣芽孢杆菌。选择季也蒙酵母、长枝木霉分别与BFA02、BFA03复配发酵生产黄腐酸。结果表明,复合菌剂发酵生产黄腐酸产量高于单菌发酵。其中,由BFA02、BFA03和长枝木霉组成的复合菌剂3实验组黄腐酸产量最高,达到103.19g/kg,较BFA02、BFA03单菌发酵分别提高了136%和74%,其纤维素、半纤维素、木质素的降解率分别为46.25%、39.49%、37.5%,均高于单菌发酵。黄腐酸生产菌株与长枝木霉复配,能够有效促进木质纤维素降解率,提高黄腐酸产量,在果渣废弃物资源化利用领域有着极大的潜力。
Fulvic acid can be produced by fruit residues to valorize agricultural organic solid wastes.Four thermoduric strains were isolated from biohumic substances.Strains BFA02 and BFA03 were screened based on their fulvic acid yield and lignocellulose decomposition.Their fulvic acid production reached 43.67 g/kg and 59.35 g/kg,respectively.Based on 16S rDNA,BFA02 and BFA03 were identified as Bacillus amyloliquefaciens and Bacillus licheniformis,respectively.The fulvic acid production by combining BFA02,BFA03 and Trichoderma longibrachiatum reached 103.19 g/kg,136% higher than that of strain BFA02 and 74% higher than that of strain BFA03.The degradation rate of cellulose,hemi cellulose and lignin were 46.25%,39.49% and 37.5%,respectively,which was higher than single strain.The fermentation efficiency of the mixed cultures was superior to that of single strain.The mixed cultures of BFA02,BFA03 and Trichoderma longibrachiatum exhibit excellent fulvic acid production and have potential applications in the resource utilization of fruit residues.