文献类型：期刊文献

英文题名：Separation and Analysis of Chlorpromazine and Demethylchlorpromazineby Capillary Electrophoresis Coupled with Electrochemiluminescence

作者：Yang, Fuxiu[1];Peng, Zhaokuai[1,2];Gu, Chunxiu[1,2];Liu, Baining[1,2];Zhou, Kaowen[1,2]

第一作者：Yang, Fuxiu

通讯作者：Zhou, KW[1];Zhou, KW[2]

机构：[1]Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China

第一机构：北京联合大学生物化学工程学院

通讯机构：[1]corresponding author), Beijing Union Univ, Biochem Engn Coll, Beijing 100023, Peoples R China;[2]corresponding author), Beijing Key Lab Biomass Waste Resource Utilizat, Beijing 100023, Peoples R China.|[1141726]北京联合大学生物化学工程学院;[11417]北京联合大学;

年份：2019

卷号：14

期号：7

起止页码：6292-6302

外文期刊名：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85071720987);WOS:【SCI-EXPANDED(收录号:WOS:000474380600030)】；

基金：This work was supported by Beijing Natural Science Foundation of China (Grant No.2152013) and State 863 Program of China (2015AA020200).

语种：英文

外文关键词：Improved capillary electrophoresis; Electrochemiluminescence; Chlorpromazine; Demethylchlorpromazine; Urine sample

摘要：Demethylchlorpromazine (DMCPZ) is one of the main metabolites of chlorpromazine (CPZ). DMCPZ has only one methyl less than CPZ. The structural difference between them is too small to separate them by conventional capillary electrophoresis (CE). However, proper addition of additives in the separation buffer can improve their separation performance. The parameters related to CE separation were carefully studied. The optimized CE conditions were separation buffer of 17.50% iso-propyl alcohol aqueous solution (v/v) containing 21 mmol/L phosphate (pH 5.3), separation voltage of 13.1 kV, sample injection time of 6 s and sample injection voltage of 11.5 kV. Combined with electrochemiluminescence (CEL) detection technology, a separation and analysis method of CPZ and DMCPZ by improved CE coupled with end-column CEL was established. The limits of detection (3 sigma) of this method were 8.1 x 10(-7) mg/mL for CPZ and 8.9 x 10(-6) mg/mL for DMCPZ. The relative standard deviations were less than 2.5% for ECL intensity and less than 1.2% for migration time. This method had many advantages, such as fast speed, high sensitivity, small sample injection, and free from interference, and was successfully applied to simultaneously determine CPZ and DMCPZ in whole urine sample of pet dog.