文献类型：期刊文献

中文题名：挤压伤后脊髓腹角和背角中神经营养因子-3和神经营养因子-4表达的时间演变规律(英文)

英文题名：Time variation of the expressions of neurotrophin-3 and neurotrophin-4 in ventral and dorsal horns of spinal cord after crushed injury

作者：董晓霞[1];张小平[2];李明[2];李雪雁[2]

机构：[1]北京联合大学继续教育学院;[2]昆明医学院解剖教研室

第一机构：北京联合大学继续教育学院

年份：2005

卷号：9

期号：29

起止页码：225-227

中文期刊名：中国临床康复

外文期刊名：Chinese Journal of Clinical Rehabilitation

收录：北大核心:【北大核心2004】；CSCD:【CSCD_E2011_2012】；

基金：云南省自然科学基金(2000C0057M);云南省教委基金资助(0012012)~~

语种：中文

中文关键词：脊髓损伤;创伤和损伤;神经组织蛋白质类

摘要：背景:神经营养因子-3和神经营养因子-4对体内、外神经元的发育、存活及功能维持具有重要的作用,是否对在挤压伤后脊髓神经元有保护和修复作用?目的:观察脊髓挤压伤模型大鼠伤后不同时间脊髓腹角和背角神经元神经营养因子-3和神经营养因子-4的表达,并分析其变化规律。设计:随机对照实验,单因素方差分析。单位:北京联合大学继续教育学院,昆明医学院人体解剖学教研室。材料:实验于2003-01/03在昆明医学院神经科学研究所完成,选择成年SD大鼠24只,随机分为4组,对照组,挤压伤24h组,挤压伤7d组及挤压伤21d组,每组6只。方法:挤压伤各组大鼠麻醉后,在T11行椎板切开后挤压大鼠T13脊髓节段,分别于术后24h,7d及21d断头处死动物,迅速取脊髓L1-L3节段制作20μm厚冰冻横切片。对照组不进行任何处理,和挤压伤24h组同时间点处死大鼠,制备切片过程相同。观察神经营养因子-3和神经营养因子-4样免疫反应阳性细胞在成年大鼠脊髓腹角和背角的分布,并计数两者相同面积内腹角和背角阳性有核细胞数。主要观察指标:①神经营养因子-3和神经营养因子-4在各组大鼠脊髓腹角和背角的分布。②神经营养因子-3和神经营养因子-4在各组大鼠脊髓腹角和背角神经元数量。结果:24只大鼠全部进入结果分析。①神经营养因子-3免疫阳性反应物主要在胞核着色,神经营养因子-4则胞浆及胞核均着色。②各组大鼠脊髓腹角和背角神经营养因子-3阳性神经元数量:挤压伤7d组和挤压伤21d组腹角阳性神经元数明显高于对照组和挤压伤24h组犤10.2±1.1,11.4±3.2,6.2±1.8,7.4±2.4,(P<0.01)犦;背角阳性神经元数仅挤压伤21d组高于对照组犤86.4±9.8,71.3±8.3,(P<0.01)犦,而挤压伤24h组及7d组低于对照组犤48.5±5.1,41.5±3.7,71.3±8.3,(P<0.01)犦。③各组大鼠脊髓腹角和背角神经营养因子-4阳性神经元数量:挤压伤24h组,挤压伤7d组和挤压伤21d组的腹角阳性神经元数高于对照组犤9.4±2.8,10.8±2.7,15.1±4.0,(P<0.05)犦,并且随时间的延长呈增加趋势(P<0.05);挤压伤7d组和挤压伤21d组背角的阳性神经元数较对照组和挤压伤24h组显著增加犤28.1±3.1,35.1±4.4,23.3±2.3,24.1±1.8,(P<0.01)犦,亦有随时间的延长呈增加趋势(P<0.01)。结论:脊髓挤压伤后,神经营养因子-3和神经营养因子-4神经元数量在脊髓腹、背角神经元中均有增加,但随时间的变化规律不完全一致,提示神经营养因子-3和神经营养因子-4在脊髓损伤修复中,对感觉和运动神经元发挥作用的时间可能不同.
BACKGROUND： Neurotrophin-3 （NT3） and neurotrophin-4 （NT4） play an important role in the growth, survival and functions of neurons in vivo or in vitro. However, it has not been reported whether they can protect and repair neurons of spinal cord after crushed spinal cord injury （cSCI）. OBJECTIVE： To explore the expression of NT3 and NT4 in ventral and dorsal horns of spinal cord at various time points after cSCI and analyze the pattern of expression changes. DESIGN： Randomized controlled study and single analysis of variance. SETTING： Continuing Education College of Beijing Union University; Department of Anatomy, Kunming Medical College. MATERIALS： The experiment was conducted in the Institute of Neuroscience of Kunming Medical College from January to March 2003. Totally 24 adult SD rats were divided randomly into 4 groups： control group, 24-hour eSCI group, 7-day cSCI group, and 21-day cSCI group with 6 rats in each group. METHODS： After anesthesia, rats in each cSCI group were dissected at vertebra TH and crushed at spinal cord T13. After 24 hours, 7 days and 21 days, the rats were killed respectively by cutting their heads off, and spinal cord L1-L3 was taken out to make frozen transactional slices of 20μm. Rats in control group received no treatment and were killed at the same time point as those in 24-hour cSC1 group, and the process of slice making was the same as mentioned above. The distribution of NT3 and NT4 positive cells in the spinal cord ventral and dorsal horns of adult rats was observed, and NT3 and NT4 positive nucleus in the ventral and dorsal horns of the same areas were counted. MAIN OUTCOME MEASURES：① Distribution of NT3 and NT4 in the rats＇ spinal cord ventral and dorsal horns; ② Number of NT3 and NT4 neurons in the rats＇ spinal cord ventral and dorsal horns. RESULTS： All the 24 rats entered the final analysis. ① NT3 positive products were mainly stained in nucleus while NT4 was stained in cytoplasm and nucleus. ② Number of NT3 neurons in ventral and dorsal horns of spinal cord in each group： The number of positive neurons in the ventral horn was significantly greater in 7-day and 21-day cSCI groups than in control and 24-hour cSC1 groups （10.2±1.1, 11.4±3.2, 6.2±1.8, 7.4 ±2.4, P 〈 0.01）. The number of positive neurons in the dorsal horn in 21day group was significantly greater than that in control group （86.4±9.8, 71.3±8.3, P 〈 0.01）; however, it was remarkably lower in 24-hour and 7- day cSCI groups than in control group （48.5± 5.1, 41.5±3.7, 71.3± 8, P 〈 0.01）. ③ Number of NT4 neurons in ventral and dorsal horns of spinal cord in each group： The number of positive neurons in the ventral horn was significantly higher in 24-hour, 7-day and 21-day eSCI groups than in control group （9.4±2.8, 10.8±2.7, 15.1±4.0, P 〈 0.05）; and it assumed an increasing tendency as time went by （P 〈 0.05）. By contrast, the number of positive neurons in the dorsal horn in 7-day and 21-day groups was significantly higher than in control and 24-hour groups （28.1 ±3.1, 35.1± 4.4, 23.3±2.3, 24.1±1.8, P 〈 0.01）, and it would increase with time following cSCI （P 〈 0.01）. CONCLUSION： The number of NT3 and NT4 positive neurons increasesin spinal cord ventral and dorsal horns after cSCI, suggesting that endogenous NT3 and NT4 have effect at different time on sensory and motor neurons in the repair of spinal cord injury.