文献类型：期刊文献

中文题名：单糖的高效毛细管区带电泳定量分析

英文题名：Determination of Monosaccharides by Capillary Zone Electrophoresis

作者：常理文[1];腰锐锋[1];陈义[1];孔捷[2]

第一作者：常理文

机构：[1]中国科学院化学研究所;[2]北京联合大学化学工程学院

第一机构：中国科学院化学研究所,北京100080

年份：1994

卷号：22

期号：1

起止页码：1-5

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytieal Chemistry

收录：CSTPCD;;Scopus;北大核心:【北大核心1992】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助课题

语种：中文

中文关键词：单糖;萘胺;毛细管电泳

外文关键词：Capillary zone electrophoresis, Monosaccharides, α-Naphthylamine

摘要：生物体中常见的9种单糖经α-萘胺衍生化后,用50mmol/L硼砂作为电泳介质,实现了高效毛细管电泳分离,并以β-吲哚乙酸为内标,研究了9种单糖毛细管电泳的定量方法,结果表明浓度在0.2～4.0mmol/L范围内呈良好的线性。6次进样的标准偏差小于±3％
Reducing monosaccharides were converted into α-naphthylamine derivatives and separated as their borate complexes by capillary zone electrophoresis using a capillary tube (25 μm i. d. 55 cm) of fused silica containing 50 mmol/L borax buffer (pH=10. 5) as car rier. The derivatives of 9 monosaccharides were completely separated in circa 20 min. The detector used is an on-column UV monitoring at 206 nm. The determination of each monosaccharide was carried out using β-indolyIacetic acid as the internal standard. A good linear relationship between the ratio of peak height of the monosaccharide derivative and the internal standard and the concentration of the monosaccharide was obtained in the range of 0. 2-0. 4 mmol/L.