文献类型：期刊文献

中文题名：小檗碱和壳聚糖抗蔬菜病原真菌活性测定及复合膜制备

英文题名：Fungistasis of Berberine and Chitosan and Preparation of Composite Membrane

作者：阎春琦[1];葛喜珍[2];田平芳[1]

第一作者：阎春琦

机构：[1]北京化工大学生命科学与技术学院;[2]北京联合大学生物化学工程学院

第一机构：北京化工大学生命科学与技术学院,北京100029

年份：2009

期号：8

起止页码：131-133

中文期刊名：北方园艺

外文期刊名：Northern Horticulture

收录：北大核心:【北大核心2008】；

基金：北京市科协金桥工程资助项目

语种：中文

中文关键词：小檗碱;壳聚糖;复合膜;蔬菜病原真菌

外文关键词：Berberine; Chitosan; Composite membrane; Vegetable pathogenic fungi

摘要：研究了小檗碱和壳聚糖对几种常见蔬菜病原真菌的抑制活性,以及以小檗碱和壳聚糖为主料制备复合膜的方法,并测定了该膜的药物释放效果。试验表明:低浓度(0.234 mg/mL)小檗碱即可抑制辣椒炭疽病菌(Vermicularia capsici)等5种蔬菜病原真菌的生长。20 mg/mL浓度壳聚糖对番茄灰霉病菌(Botrytis cinerea)的抑制率高达65%,而对其余4种果蔬病原真菌也有一定的抑制作用。为了集成这2种天然化合物的优点,制备了小檗碱-壳聚糖复合膜,该膜具有缓释功能,在模拟外部环境(磷酸缓冲液,pH 6.8)条件下,20 d小檗碱累计释放率接近25%,提示其在果蔬贮藏抗菌中的应用价值。
The antifungal activities of berberine and chitosan were studied by growth rate method. It was found that berberine of low concentration （0. 234 mg/mL） could inhibit the mycelial growth of vegetable pathogenic fungi, including Alternaria solani , Vermicularia capsici , Botrytis cinerea , Fusarium oxysporum f. sp. cucumerium, and Fusarium oxysporam f. sp. melonis. Another natural compound, ehitosan also inhibited their mycelial growth. Chitosan showed approximately 65% inhibition rate against B. cinerea. To integrate the two naturally occurring compounds, berberine-chitosan composite membrane was thus prepared and in vitro experiment revealed the berberine slow release, suggesting its potential utilization in controlling vegetable storage diseases.